TY - JOUR
T1 - Optimisation of XAD extraction methodology for the assessment of biodegradation potential of 14C-phenanthrene in soil
AU - Adedigba, Bilqees M.
AU - OGBONNAYA, Ogbonnaya Uchenna
AU - Vázquez-Cuevas, Gabriela M.
AU - Semple, Kirk T.
N1 - Funding Information:
The authors would like to give thanks to the UK Department for International Development (DFID) NGCS-2009-245 for funding this project through a doctoral scholarship administered by the Association of Commonwealth Universities (ACU). GVC would like to thank the Mexican National Council for Science and Technology (CONACyT) for their financial support through a scholarship (No. 313807 ) to pursue postgraduate studies at the Lancaster Environment Centre, Lancaster University.
Funding Information:
The authors would like to give thanks to the UK Department for International Development (DFID)NGCS-2009-245 for funding this project through a doctoral scholarship administered by the Association of Commonwealth Universities (ACU). GVC would like to thank the Mexican National Council for Science and Technology (CONACyT) for their financial support through a scholarship (No. 313807) to pursue postgraduate studies at the Lancaster Environment Centre, Lancaster University.
PY - 2018/2
Y1 - 2018/2
N2 - This study investigated the use of a hydrophobic resin, amberlite XAD, as a tool for assessing the biodegradation potential of 14C-phenanthrene in soil. The method was optimised in terms of soil/XAD ratio, shaking, extraction time and eluting solvent. The most effective method was then tested on selected XADs, and the performance compared with cyclodextrin (HP-β-CD) and dichloromethane (DCM) extractions suitability to predict phenanthrene biodegradation in soil over 100 d. Results showed that the optimum conditions for the XAD extraction technique are a 2:1 soil/XAD ratio, 100 rpm mixing for 22 h and elution using a DCM:methanol solution (1:1). Mineralisation of 14C-phenanthrene was accurately predicted by HP-β-CD (r2=0.990, slope = 0.953, intercept = 1.374) and XAD-4 extractions (r2=0.989, slope = 0.820, intercept = 6.567), while DCM overestimated the bioaccessibility of 14C-phenanthrene (r2=0.999, slope = 1.328, intercept =−49.507). This investigation showed that XAD extraction can be considered a suitable non-exhaustive technique for estimating biodegradability of phenanthrene in soil.
AB - This study investigated the use of a hydrophobic resin, amberlite XAD, as a tool for assessing the biodegradation potential of 14C-phenanthrene in soil. The method was optimised in terms of soil/XAD ratio, shaking, extraction time and eluting solvent. The most effective method was then tested on selected XADs, and the performance compared with cyclodextrin (HP-β-CD) and dichloromethane (DCM) extractions suitability to predict phenanthrene biodegradation in soil over 100 d. Results showed that the optimum conditions for the XAD extraction technique are a 2:1 soil/XAD ratio, 100 rpm mixing for 22 h and elution using a DCM:methanol solution (1:1). Mineralisation of 14C-phenanthrene was accurately predicted by HP-β-CD (r2=0.990, slope = 0.953, intercept = 1.374) and XAD-4 extractions (r2=0.989, slope = 0.820, intercept = 6.567), while DCM overestimated the bioaccessibility of 14C-phenanthrene (r2=0.999, slope = 1.328, intercept =−49.507). This investigation showed that XAD extraction can be considered a suitable non-exhaustive technique for estimating biodegradability of phenanthrene in soil.
KW - Bioaccessibility
KW - HP-β-CD
KW - Hydrophobic resins
KW - XAD
UR - http://www.scopus.com/inward/record.url?scp=85037647020&partnerID=8YFLogxK
U2 - 10.1016/j.eti.2017.11.011
DO - 10.1016/j.eti.2017.11.011
M3 - Journal article
AN - SCOPUS:85037647020
SN - 2352-1864
VL - 9
SP - 140
EP - 150
JO - Environmental Technology and Innovation
JF - Environmental Technology and Innovation
ER -