Abstract
Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique.
| Original language | English |
|---|---|
| Pages (from-to) | 42-48 |
| Number of pages | 7 |
| Journal | Methods |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 May 2003 |
User-Defined Keywords
- Biosensors
- Fluorescence resonance energy transfer
- Green fluorescent protein
- Optical imaging
- Voltage-sensitive dyes
