Optical Aspartame Biosensor Based on Bienzyme Immobilisation on Egg Shell Membrane

Xiao Dan, Martin M. F. Choi

Research output: Contribution to journalJournal article


Aspartame is a widely used artificial sweetener in many low calorie foods and beverages. Determination of its concentration in foodstuff is important as aspartame is a relatively expensive material. A novel optical biosensor for the determination of aspartame in beverage based on a bienzyme system is presented. An aspartame biosensor was fabricated employing a bienzyme system composed of chymotrypsin and alcohol oxidase immobilised onto a egg shell membrane and an oxygen-sensitive optode membrane as the transducer. The detection schemes involve the enzymatic reactions of aspartame leading to the depletion of oxygen level of the medium with concomitant enhancement of the fluorescence intensity of the oxygen-sensitive membrane.
Original languageEnglish
Pages (from-to)486
Number of pages1
JournalChemical Journal of Chinese Universities
Issue numberS1
Publication statusPublished - May 1999


Dive into the research topics of 'Optical Aspartame Biosensor Based on Bienzyme Immobilisation on Egg Shell Membrane'. Together they form a unique fingerprint.

Cite this