TY - JOUR
T1 - Online concentration of aristolochic acid I and II in Chinese medicine preparations by micellar electrokinetic chromatography
AU - Shi, Shenhua
AU - Li, Wei
AU - Liao, Yiping
AU - CAI, Zongwei
AU - Liu, Huwei
N1 - Funding Information:
This study is financially supported by NSFC, Grant Nos. 20575003 and 20635001. The authors would like to acknowledge Agilent Technologies for providing the HP 3D CE system.
PY - 2007/10/5
Y1 - 2007/10/5
N2 - In this study, an online concentration method in micellar electrokinetic chromatography (MEKC) applying field-enhanced sample injection (FESI) mode was developed for the detection of aristolochic acids (AAs) in Chinese medicine preparations. AA-I and AA-II were baseline separated with high separation efficiency, and 100-fold enhancement of the detection sensitivity was achieved compared with those obtained from normal capillary zone electrophoresis (CZE) or simple MEKC method. The proposed method was successfully applied for the determination of AAs in Chinese medicine preparations.
AB - In this study, an online concentration method in micellar electrokinetic chromatography (MEKC) applying field-enhanced sample injection (FESI) mode was developed for the detection of aristolochic acids (AAs) in Chinese medicine preparations. AA-I and AA-II were baseline separated with high separation efficiency, and 100-fold enhancement of the detection sensitivity was achieved compared with those obtained from normal capillary zone electrophoresis (CZE) or simple MEKC method. The proposed method was successfully applied for the determination of AAs in Chinese medicine preparations.
KW - Aristolochic acid
KW - Chinese herbs nephropathy
KW - Chinese medicine preparations
KW - Field-enhanced sample injection
KW - Online concentration
KW - Reversed electrode polarity stacking mode
UR - http://www.scopus.com/inward/record.url?scp=34548537676&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2007.08.023
DO - 10.1016/j.chroma.2007.08.023
M3 - Journal article
C2 - 17765252
AN - SCOPUS:34548537676
SN - 0021-9673
VL - 1167
SP - 120
EP - 124
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -