Abstract
BACKGROUND: Aflatoxins, a kind of carcinogen, have attracted increasing attention due to their toxicity and harmfulness to human health. Traditional methods for aflatoxins analysis usually involve tedious extraction steps with a subsequent derivatization process. Herein, a simple and efficient liquid-phase microextraction method based on deep eutectic solvents (DESs) for direct analysis of aflatoxins was developed. RESULTS: Adopting DESs as the extractant, we surprisingly found out that DESs could either achieve good extraction performance or play a similar role to the derivatization agent, achieving an enhancement of fluorescence intensity for direct analysis of aflatoxins by high-performance liquid chromatography combined with fluorescent detection. Under optimal conditions obtained by response surface methodology, the method provided satisfactory linear ranges (0.01–0.75 μg kg−1 for AFB1 and AFG1, 0.003–0.25 μg kg−1 for AFB2 and AFG2) with good determination coefficients (R2 > 0.9988), a low detection limit (0.0005–0.003 μg kg−1), and good recovery rates (72.05–113.54%). CONCLUSION: These results highlighted superiorities of the one-step DES strategy for analysis of aflatoxins in edible oils, providing insights for future development of efficient methods in food analysis.
Original language | English |
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Pages (from-to) | 4840-4848 |
Number of pages | 9 |
Journal | Journal of the Science of Food and Agriculture |
Volume | 100 |
Issue number | 13 |
Early online date | 1 Jun 2020 |
DOIs | |
Publication status | Published - Oct 2020 |
Scopus Subject Areas
- Biotechnology
- Food Science
- Agronomy and Crop Science
- Nutrition and Dietetics
User-Defined Keywords
- aflatoxins
- deep eutectic solvents
- edible oils
- liquid-phase microextraction