One-step deep eutectic solvent strategy for efficient analysis of aflatoxins in edible oils

Tingting He, Tong Zhou, Hao Wan, Simon Q B HAN, Yaqian Ma, Ting Tan, Yiqun Wan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Aflatoxins, a kind of carcinogen, have attracted increasing attention due to their toxicity and harmfulness to human health. Traditional methods for aflatoxins analysis usually involve tedious extraction steps with a subsequent derivatization process. Herein, a simple and efficient liquid-phase microextraction method based on deep eutectic solvents (DESs) for direct analysis of aflatoxins was developed. RESULTS: Adopting DESs as the extractant, we surprisingly found out that DESs could either achieve good extraction performance or play a similar role to the derivatization agent, achieving an enhancement of fluorescence intensity for direct analysis of aflatoxins by high-performance liquid chromatography combined with fluorescent detection. Under optimal conditions obtained by response surface methodology, the method provided satisfactory linear ranges (0.01–0.75 μg kg−1 for AFB1 and AFG1, 0.003–0.25 μg kg−1 for AFB2 and AFG2) with good determination coefficients (R2 > 0.9988), a low detection limit (0.0005–0.003 μg kg−1), and good recovery rates (72.05–113.54%). CONCLUSION: These results highlighted superiorities of the one-step DES strategy for analysis of aflatoxins in edible oils, providing insights for future development of efficient methods in food analysis.

Original languageEnglish
Pages (from-to)4840-4848
Number of pages9
JournalJournal of the Science of Food and Agriculture
Volume100
Issue number13
DOIs
Publication statusPublished - 1 Oct 2020

Scopus Subject Areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

User-Defined Keywords

  • aflatoxins
  • deep eutectic solvents
  • edible oils
  • liquid-phase microextraction

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