On-line flow injection-cloud point preconcentration of polycyclic aromatic hydrocarbons coupled with high-performance liquid chromatography

Cheuk Fai Li, Jonathan W C WONG, Carmen W K HUIE, Martin M F CHOI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Cloud point methodology has been used to develop a novel preconcentration and an analytical method for polycyclic aromatic hydrocarbons (PAHs) in soil sample. The nonionic surfactant Tergitol 15-S-7 was successfully used as the surfactant-mediated extractant in both ultrasonic and microwave-assisted extractions. Over 90% of recoveries for various PAHs in soil were obtained under the optimal experimental conditions. The extracts were then preconcentrated and analyzed by our proposed on-line coupling method "flow injection-cloud point preconcentration-high-performance liquid chromatography" (FI-CPP-HPLC) equipped with a fluorescence detector and an excitation/emission wavelength program. The preconcentration system and optimal working conditions were established. The limit of detection of the FI-CPE-HPLC system ranges from 0.101 to 0.456 μg/L for the selected PAHs, i.e., phenanthrene, pyrene, chrysene, benzo[k]fluoranthene and benzo[a]pyrene. Our proposed technique provides a reliable, simple and automatic analytical method for the determination of PAHs in environmental soil samples.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalJournal of Chromatography A
Volume1214
Issue number1-2
DOIs
Publication statusPublished - 19 Dec 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Cloud point extraction
  • Polycyclic aromatic hydrocarbons
  • Preconcentration
  • Soil

Fingerprint

Dive into the research topics of 'On-line flow injection-cloud point preconcentration of polycyclic aromatic hydrocarbons coupled with high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this