Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale

Tin Long Wong, Li Feng Li*, Ji Xia Zhang, Quan Wei Zhang, Xue Ting Zhang, Li Shuang Zhou, Hau Yee Fung, Lei Feng, Hui Yuan Cheng, Chu Ying Huo, Man Liu, Wan Rong Bao, Wen Jie Wu, Cheuk Hei Lai, Su Ping Bai, Shao Ping Nie, Pema Tenzin Puno, Clara Bik San Lau, Ping Chung Leung, Quan Bin Han*Han Dong Sun

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

10 Citations (Scopus)


The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.

Original languageEnglish
Article number108038
Number of pages13
JournalFood Hydrocolloids
Early online date4 Aug 2022
Publication statusPublished - Jan 2023

Scopus Subject Areas

  • Food Science
  • Chemistry(all)
  • Chemical Engineering(all)

User-Defined Keywords

  • Dendrobium officinale polysaccharide
  • Oligosaccharide
  • Structure analysis
  • β-1,4-Mannan


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