Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale

Tin Long Wong; Li Feng Li; Ji Xia Zhang; Quan Wei Zhang; Xue Ting Zhang; Li Shuang Zhou; Hau Yee Fung; Lei Feng; Hui Yuan Cheng; Chu Ying Huo; Man Liu; Wan Rong Bao; Wen Jie Wu; Cheuk Hei Lai; Su Ping Bai; Shao Ping Nie; Pema Tenzin Puno; Clara Bik San Lau; Ping Chung Leung; Quan Bin Han; Han Dong Sun

doi:10.1016/j.foodhyd.2022.108038

Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale

Tin Long Wong, Li Feng Li^*, Ji Xia Zhang, Quan Wei Zhang, Xue Ting Zhang, Li Shuang Zhou, Hau Yee Fung, Lei Feng, Hui Yuan Cheng, Chu Ying Huo, Man Liu, Wan Rong Bao, Wen Jie Wu, Cheuk Hei Lai, Su Ping Bai, Shao Ping Nie, Pema Tenzin Puno, Clara Bik San Lau, Ping Chung Leung, Quan Bin Han^*Han Dong Sun

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

20 Citations (Scopus)

Abstract

The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.

Original language	English
Article number	108038
Number of pages	13
Journal	Food Hydrocolloids
Volume	134
Early online date	4 Aug 2022
DOIs	https://doi.org/10.1016/j.foodhyd.2022.108038
Publication status	Published - Jan 2023

User-Defined Keywords

Dendrobium officinale polysaccharide
Oligosaccharide
Structure analysis
β-1,4-Mannan

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10.1016/j.foodhyd.2022.108038

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Wong, T. L., Li, L. F., Zhang, J. X., Zhang, Q. W., Zhang, X. T., Zhou, L. S., Fung, H. Y., Feng, L., Cheng, H. Y., Huo, C. Y., Liu, M., Bao, W. R., Wu, W. J., Lai, C. H., Bai, S. P., Nie, S. P., Puno, P. T., Lau, C. B. S., Leung, P. C., ... Sun, H. D. (2023). Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale. Food Hydrocolloids, 134, Article 108038. https://doi.org/10.1016/j.foodhyd.2022.108038

@article{07710be79b5140c183773c9ce9dd0939,

title = "Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale",

abstract = "The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.",

keywords = "Dendrobium officinale polysaccharide, Oligosaccharide, Structure analysis, β-1,4-Mannan",

author = "Wong, {Tin Long} and Li, {Li Feng} and Zhang, {Ji Xia} and Zhang, {Quan Wei} and Zhang, {Xue Ting} and Zhou, {Li Shuang} and Fung, {Hau Yee} and Lei Feng and Cheng, {Hui Yuan} and Huo, {Chu Ying} and Man Liu and Bao, {Wan Rong} and Wu, {Wen Jie} and Lai, {Cheuk Hei} and Bai, {Su Ping} and Nie, {Shao Ping} and Puno, {Pema Tenzin} and Lau, {Clara Bik San} and Leung, {Ping Chung} and Han, {Quan Bin} and Sun, {Han Dong}",

note = "Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF) , Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14, 2019-2-06 ), Health Medical Research Fund ( 11122531 , 14150521 , 17182681 ), National Natural Sciences Foundation in China ( 81473341 , 82173948 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14–15/06, FRG2/17–18/060 and FRG2/16–17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2023",

month = jan,

doi = "10.1016/j.foodhyd.2022.108038",

language = "English",

volume = "134",

journal = "Food Hydrocolloids",

issn = "0268-005X",

publisher = "Elsevier",

}

Wong, TL, Li, LF, Zhang, JX, Zhang, QW, Zhang, XT, Zhou, LS, Fung, HY, Feng, L, Cheng, HY, Huo, CY, Liu, M, Bao, WR, Wu, WJ, Lai, CH, Bai, SP, Nie, SP, Puno, PT, Lau, CBS, Leung, PC, Han, QB & Sun, HD 2023, 'Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale', Food Hydrocolloids, vol. 134, 108038. https://doi.org/10.1016/j.foodhyd.2022.108038

TY - JOUR

T1 - Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale

AU - Wong, Tin Long

AU - Li, Li Feng

AU - Zhang, Ji Xia

AU - Zhang, Quan Wei

AU - Zhang, Xue Ting

AU - Zhou, Li Shuang

AU - Fung, Hau Yee

AU - Feng, Lei

AU - Cheng, Hui Yuan

AU - Huo, Chu Ying

AU - Liu, Man

AU - Bao, Wan Rong

AU - Wu, Wen Jie

AU - Lai, Cheuk Hei

AU - Bai, Su Ping

AU - Nie, Shao Ping

AU - Puno, Pema Tenzin

AU - Lau, Clara Bik San

AU - Leung, Ping Chung

AU - Han, Quan Bin

AU - Sun, Han Dong

N1 - Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF) , Tier 3, ITS/311/09 , General Research Fund ( 12100615 , 22100014 , 12100818 ), UGC Research Matching Grant Scheme ( 2019-1-10 , 2019-1-14, 2019-2-06 ), Health Medical Research Fund ( 11122531 , 14150521 , 17182681 ), National Natural Sciences Foundation in China ( 81473341 , 82173948 ), the Science and Technology Project of Shenzhen ( JCYJ20160531193812867 ), the Key-Area Research and Development Program of Guangdong Province ( 2020B1111110007 ), Hong Kong Baptist University (RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14–15/06, FRG2/17–18/060 and FRG2/16–17/002 ), and Vincent & Lily Woo Foundation . Publisher Copyright: © 2022 The Authors

PY - 2023/1

Y1 - 2023/1

N2 - The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.

AB - The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data.

KW - Dendrobium officinale polysaccharide

KW - Oligosaccharide

KW - Structure analysis

KW - β-1,4-Mannan

UR - http://www.scopus.com/inward/record.url?scp=85135867905&partnerID=8YFLogxK

U2 - 10.1016/j.foodhyd.2022.108038

DO - 10.1016/j.foodhyd.2022.108038

M3 - Journal article

SN - 0268-005X

VL - 134

JO - Food Hydrocolloids

JF - Food Hydrocolloids

M1 - 108038

ER -

Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale

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