Off-line comprehensive two-dimensional high-performance liquid chromatography system with size exclusion column and reverse phase column for separation of complex traditional Chinese medicine Qingkailing injection

Sai Ma, Lingxin Chen, Guoan Luo*, Kangning REN, Juanfang Wu, Yiming Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

A comprehensive two-dimensional liquid chromatography system was constructed on the combination of size exclusion chromatography (SEC) and reverse phase liquid chromatography (RPLC). The first dimension used a SEC column with 300 mm × 8 mm i.d., packed with Toyopearl HW-40S. The column was eluted with 0.05 mol/l Tris-HCl (pH 6.9) at a flow rate of 0.4 ml/min. The second dimension used a RPLC column with 100 mm × 4.6 mm i.d., which was operated in gradient form at a flow rate of 1.0 ml/min. An automatic switching valve was used to collect the effluent of the SEC column every 3 min, and the effluent was automatically injected into the RPLC column. Mass spectrometer was used for peak identification. This system was used to separate Qingkailing injection, a traditional Chinese medicine (TCM). The result showed that the total peak capacity of this system could reach 1134 and the qualitative analysis of seven chemical components of the Qingkailing injection was accomplished by this system. The results show that comprehensive two-dimensional liquid chromatography system is of great importance and high value in the separation of complex TCM.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalJournal of Chromatography A
Volume1127
Issue number1-2
DOIs
Publication statusPublished - 15 Sep 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Comprehensive two-dimensional liquid chromatography
  • Mass spectrometer
  • Peak capacity
  • Qingkailing injection
  • Traditional Chinese medicine

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