Nuclear patch-clamp electrophysiology of Ca2+ channels

Don On Daniel Mak, Horia Vais, King Ho Cheung, J. Kevin Foskett*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.
Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalCold Spring Harbor Protocols
Volume9
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

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