Nuclear patch-clamp electrophysiology of Ca2+ channels

Don On Daniel Mak; Horia Vais; King Ho Cheung; J. Kevin Foskett

doi:10.1101/pdb.prot073064

Nuclear patch-clamp electrophysiology of Ca2+ channels

Don On Daniel Mak, Horia Vais, King Ho Cheung, J. Kevin Foskett^*

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

13 Citations (Scopus)

Abstract

Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.

Original language	English
Pages (from-to)	885-891
Number of pages	7
Journal	Cold Spring Harbor Protocols
Volume	9
DOIs	https://doi.org/10.1101/pdb.prot073064
Publication status	Published - Sept 2013

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title = "Nuclear patch-clamp electrophysiology of Ca2+ channels",

abstract = "Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.",

author = "Mak, {Don On Daniel} and Horia Vais and Cheung, {King Ho} and Foskett, {J. Kevin}",

year = "2013",

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T1 - Nuclear patch-clamp electrophysiology of Ca2+ channels

AU - Mak, Don On Daniel

AU - Vais, Horia

AU - Cheung, King Ho

AU - Foskett, J. Kevin

PY - 2013/9

Y1 - 2013/9

N2 - Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.

AB - Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984033/

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84883476869&partnerID=MN8TOARS

U2 - 10.1101/pdb.prot073064

DO - 10.1101/pdb.prot073064

M3 - Journal article

SN - 1940-3402

VL - 9

SP - 885

EP - 891

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

ER -

Nuclear patch-clamp electrophysiology of Ca2+ channels

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