NRBF2 is involved in the autophagic degradation process of APP-CTFs in Alzheimer disease models

Chuanbin Yang, Cui Zan Cai, Juxian Song, Jie Qiong Tan, S S Kumar Durairajan, Ashok Iyaswamy, Ming Yue Wu, Lei Lei Chen, Zhenyu Yue, Min Li*, Jia Hong Lu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

55 Citations (Scopus)
27 Downloads (Pure)


Alzheimer disease (AD) is the most common neurodegenerative disease characterized by the deposition of amyloid plaque in the brain. The autophagy-associated PIK3C3-containing phosphatidylinositol 3-kinase (PtdIns3K) complex has been shown to interfere with APP metabolism and amyloid beta peptide (Aβ) homeostasis via poorly understood mechanisms. Here we report that NRBF2 (nuclear receptor binding factor 2), a key component and regulator of the PtdIns3K, is involved in APP-CTFs homeostasis in AD cell models. We found that NRBF2 interacts with APP in vivo and its expression levels are reduced in hippocampus of 5XFAD AD mice; we further demonstrated that NRBF2 overexpression promotes degradation of APP C-terminal fragments (APP-CTFs), and reduces Aβ1–40 and Aβ1-42 levels in human mutant APP-overexpressing cells. Conversely, APP-CTFs, Aβ1–40 and Aβ1-42 levels were increased in Nrbf2 knockdown or nrbf2 knockout cells. Furthermore, NRBF2 positively regulates autophagy in neuronal cells and NRBF2-mediated reduction of APP-CTFs levels is autophagy dependent. Importantly, nrbf2 knockout attenuates the recruitment of APP and APP-CTFs into phagophores and the sorting of APP and APP-CTFs into endosomal intralumenal vesicles, which is accompanied by the accumulation of the APP and APP-CTFs into RAB5-positive early endosomes. Collectively, our results reveal the potential connection between NRBF2 and the AD-associated protein APP by showing that NRBF2 plays an important role in regulating degradation of APP-CTFs through modulating autophagy.

Original languageEnglish
Pages (from-to)2028-2040
Number of pages13
Issue number12
Publication statusPublished - 2 Dec 2017

Scopus Subject Areas

  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • Alzheimer's disease
  • APP
  • autophagy
  • class III phosphatidylinositol 3-kinase (PtdIns3K)
  • NRBF2


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