Novel composites of multifunctional Fe3O4@Au nanofibers for highly efficient glycoprotein imprinting

Yanxia Li, Mei Hong, Miaomiao, Qiu Bin, Zhenyu Lin, Zongwei CAI*, Guonan Chen

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

76 Citations (Scopus)


A kind of surface glycoprotein imprinting over magnetic Fe 3O4@Au multifunctional nanofibers (NFs) was developed and investigated. Magnetic Fe3O4@Au nanoparticles (NPs) as the core materials were modified consecutively with aniline, 3-aminophenylboronic acid (APBA) and acrylic acid to introduce boronic acids and polymerizable double bonds. With horseradish peroxidase (HRP) as a glycoprotein template, thin protein-imprinted films were fabricated via radical induced graft copolymerization of monomers on the surface of the multifunctional NFs. Experimental results show that the magnetic multifunctional Fe3O 4@Au NFs can not only direct the selective occurrence of imprinting polymerization, but also drive glycoprotein templates into the polymer through reversible covalent complex formation. The results show that the imprinted NFs reached saturated adsorption at 0.3 mg mL-1 within 90 min and exhibited significant specific recognition towards the template protein. Moreover, the molecularly imprinted polymers (MIPs) were used to electrochemically detect HRP with good linearity in the range of low concentrations from 0.01 to 0.30 mg mL-1 through molecular recognition of K3[Fe(CN)6] current response. The detection limit of this method was found to be 0.005 mg mL-1 (S/N = 3). The synthetic strategy paves the way for preparation of functional nanomaterials by molecular imprinting technique and direct detection of proteins in a more convenient, simpler and cheaper way.

Original languageEnglish
Pages (from-to)1044-1051
Number of pages8
JournalJournal of Materials Chemistry B
Issue number7
Publication statusPublished - 21 Feb 2013

Scopus Subject Areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)


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