@article{71f8b5e3ae674a7db0184e23cf3db65b,
title = "Non-cryogenic structure of a chloride pump provides crucial clues to temperature-dependent channel transport efficiency",
abstract = "Non-cryogenic protein structures determined at ambient temperature may disclose significant information about protein activity. Chloride-pumping rhodopsin (ClR) exhibits a trend to hyperactivity induced by a change in the photoreaction rate because of a gradual decrease in temperature. Here, to track the structural changes that explain the differences in CIR activity resulting from these temperature changes, we used serial femtosecond crystallography (SFX) with an X-ray free electron laser (XFEL) to determine the non-cryogenic structure of ClR at a resolution of 1.85 {\AA}, and compared this structure with a cryogenic ClR structure obtained with synchrotron X-ray crystallography. The XFEL-derived ClR structure revealed that the all-trans retinal (ATR) region and positions of two coordinated chloride ions slightly differed from those of the synchrotron-derived structure. Moreover, the XFEL structure enabled identification of one additional water molecule forming a hydrogen bond network with a chloride ion. Analysis of the channel cavity and a difference distance matrix plot (DDMP) clearly revealed additional structural differences. B-factor information obtained from the non-cryogenic structure supported a motility change on the residual main and side chains as well as of chloride and water molecules because of temperature effects. Our results indicate that non-cryogenic structures and time-resolved XFEL experiments could contribute to a better understanding of the chloride-pumping mechanism of ClR and other ion pumps.",
author = "Ji-Hye Yun and Xuanxuan Li and Jae-Hyun Park and Yang Wang and Mio Ohki and Zeyu Jin and Wonbin Lee and Sam-yong Park and Hao Hu and Chufeng Li and Nadia Zatsepin and Hunter, {Mark S.} and Sierra, {Raymond G.} and Jake Koralek and Yoon, {Chun Hong} and Hyun-Soo Cho and Uwe Weierstall and Leihan Tang and Haiguang Liu and Weontae Lee",
note = "Acknowledgments: We are grateful to the staff scientists at the Coherent X-ray Imaging (CXI) station of the Linac Coherent Light Source of the SLAC National Accelerator Laboratory, BL17A beamline of the Photon Factory. We also thank the staff scientists at the Pohang Accelerator Laboratory X-ray Free Electron Laser (PAL-XFEL) for their technical support on the initial screening. This research was supported by a Tianhe-2JK computing time award at the Beijing Computational Research Center (CSRC). Portions of this research were carried out at the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory. This LCLS beam time was part of the Protein Crystal Screening (PCS) program. LCLS is an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University. Use of the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. Parts of the sample delivery system used at LCLS for this research were funded by the National Institutes of Health Grant P41GM103393, formerly P41RR001209.",
year = "2019",
month = jan,
doi = "10.1074/jbc.RA118.004038",
language = "English",
volume = "294",
pages = "794--804",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.; Elsevier",
number = "3",
}