Nitric oxide mediates brassinosteroid-induced ABA biosynthesis involved in oxidative stress tolerance in maize leaves

Aying Zhang; Jun Zhang; Jianhua ZHANG; Nenghui Ye; Hong Zhang; Mingpu Tan; Mingyi Jiang

doi:10.1093/pcp/pcq187

Nitric oxide mediates brassinosteroid-induced ABA biosynthesis involved in oxidative stress tolerance in maize leaves

Aying Zhang, Jun Zhang, Jianhua ZHANG, Nenghui Ye, Hong Zhang, Mingpu Tan, Mingyi Jiang^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

156 Citations (Scopus)

Abstract

The role of ABA in brassinosteroid (BR)-induced stress tolerance and the relationship between BR, nitric oxide (NO) and ABA under water stress induced by polyethylene glycol (PEG) were investigated in leaves of maize (Zea mays) plants. Water stress led to oxidative damage. Pre-treatment with the BR biosynthetic inhibitor brassinazole (Brz) aggravated the oxidative damage induced by PEG treatment, which was alleviated by the application of BR or ABA. Pre-treatment with the ABA biosynthetic inhibitor fluridone also aggravated the oxidative damage induced by PEG treatment; however, this was barely alleviated by the application of BR. BR treatment increased the content of ABA and up-regulated the expression of the ABA biosynthetic gene vp14 in maize leaves, which was blocked by pre-treatments with the NO scavenger cPTIO (2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and the nitric oxide synthase inhibitor l-NAME (N^G-nitro-l-arginine methyl ester. Moreover, BR treatment induced increases in the generation of NO in mesophyll cells of maize leaves, and treatment with the NO donor sodium nitroprusside (SNP) up-regulated the content of ABA and the expression of vp14 in maize leaves. Our results suggest that BR-induced NO production and NO-activated ABA biosynthesis are important mechanisms for BR-enhanced water stress tolerance in leaves of maize plants.

Original language	English
Pages (from-to)	181-192
Number of pages	12
Journal	Plant and Cell Physiology
Volume	52
Issue number	1
DOIs	https://doi.org/10.1093/pcp/pcq187
Publication status	Published - Jan 2011

Scopus Subject Areas

Physiology
Plant Science
Cell Biology

User-Defined Keywords

ABA
BR
Maize
NO
Oxidative damage

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10.1093/pcp/pcq187

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title = "Nitric oxide mediates brassinosteroid-induced ABA biosynthesis involved in oxidative stress tolerance in maize leaves",

abstract = "The role of ABA in brassinosteroid (BR)-induced stress tolerance and the relationship between BR, nitric oxide (NO) and ABA under water stress induced by polyethylene glycol (PEG) were investigated in leaves of maize (Zea mays) plants. Water stress led to oxidative damage. Pre-treatment with the BR biosynthetic inhibitor brassinazole (Brz) aggravated the oxidative damage induced by PEG treatment, which was alleviated by the application of BR or ABA. Pre-treatment with the ABA biosynthetic inhibitor fluridone also aggravated the oxidative damage induced by PEG treatment; however, this was barely alleviated by the application of BR. BR treatment increased the content of ABA and up-regulated the expression of the ABA biosynthetic gene vp14 in maize leaves, which was blocked by pre-treatments with the NO scavenger cPTIO (2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and the nitric oxide synthase inhibitor l-NAME (NG-nitro-l-arginine methyl ester. Moreover, BR treatment induced increases in the generation of NO in mesophyll cells of maize leaves, and treatment with the NO donor sodium nitroprusside (SNP) up-regulated the content of ABA and the expression of vp14 in maize leaves. Our results suggest that BR-induced NO production and NO-activated ABA biosynthesis are important mechanisms for BR-enhanced water stress tolerance in leaves of maize plants.",

keywords = "ABA, BR, Maize, NO, Oxidative damage",

author = "Aying Zhang and Jun Zhang and Jianhua ZHANG and Nenghui Ye and Hong Zhang and Mingpu Tan and Mingyi Jiang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China [grant Nos. 30700491 and 30971712 to A.Z. and grant Nos. 30970238 and 90717108 to M.J.]; the Research Grant for Young Teachers of China from Agricultural University and Nanjing Agricultural University [grant No. NC2007005 to A.Z.]; University Grants Committee of Hong Kong [grant No. AoE/B-07/99 to J.Z.], Hong Kong Research Grants Council [grant No. HKBU 262708 to J.Z.]; the Fundamental Research Funds for the Central Universities [grant No. KYZ200905 to M.J.].",

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doi = "10.1093/pcp/pcq187",

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AU - Zhang, Hong

AU - Tan, Mingpu

AU - Jiang, Mingyi

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China [grant Nos. 30700491 and 30971712 to A.Z. and grant Nos. 30970238 and 90717108 to M.J.]; the Research Grant for Young Teachers of China from Agricultural University and Nanjing Agricultural University [grant No. NC2007005 to A.Z.]; University Grants Committee of Hong Kong [grant No. AoE/B-07/99 to J.Z.], Hong Kong Research Grants Council [grant No. HKBU 262708 to J.Z.]; the Fundamental Research Funds for the Central Universities [grant No. KYZ200905 to M.J.].

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N2 - The role of ABA in brassinosteroid (BR)-induced stress tolerance and the relationship between BR, nitric oxide (NO) and ABA under water stress induced by polyethylene glycol (PEG) were investigated in leaves of maize (Zea mays) plants. Water stress led to oxidative damage. Pre-treatment with the BR biosynthetic inhibitor brassinazole (Brz) aggravated the oxidative damage induced by PEG treatment, which was alleviated by the application of BR or ABA. Pre-treatment with the ABA biosynthetic inhibitor fluridone also aggravated the oxidative damage induced by PEG treatment; however, this was barely alleviated by the application of BR. BR treatment increased the content of ABA and up-regulated the expression of the ABA biosynthetic gene vp14 in maize leaves, which was blocked by pre-treatments with the NO scavenger cPTIO (2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and the nitric oxide synthase inhibitor l-NAME (NG-nitro-l-arginine methyl ester. Moreover, BR treatment induced increases in the generation of NO in mesophyll cells of maize leaves, and treatment with the NO donor sodium nitroprusside (SNP) up-regulated the content of ABA and the expression of vp14 in maize leaves. Our results suggest that BR-induced NO production and NO-activated ABA biosynthesis are important mechanisms for BR-enhanced water stress tolerance in leaves of maize plants.

AB - The role of ABA in brassinosteroid (BR)-induced stress tolerance and the relationship between BR, nitric oxide (NO) and ABA under water stress induced by polyethylene glycol (PEG) were investigated in leaves of maize (Zea mays) plants. Water stress led to oxidative damage. Pre-treatment with the BR biosynthetic inhibitor brassinazole (Brz) aggravated the oxidative damage induced by PEG treatment, which was alleviated by the application of BR or ABA. Pre-treatment with the ABA biosynthetic inhibitor fluridone also aggravated the oxidative damage induced by PEG treatment; however, this was barely alleviated by the application of BR. BR treatment increased the content of ABA and up-regulated the expression of the ABA biosynthetic gene vp14 in maize leaves, which was blocked by pre-treatments with the NO scavenger cPTIO (2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and the nitric oxide synthase inhibitor l-NAME (NG-nitro-l-arginine methyl ester. Moreover, BR treatment induced increases in the generation of NO in mesophyll cells of maize leaves, and treatment with the NO donor sodium nitroprusside (SNP) up-regulated the content of ABA and the expression of vp14 in maize leaves. Our results suggest that BR-induced NO production and NO-activated ABA biosynthesis are important mechanisms for BR-enhanced water stress tolerance in leaves of maize plants.

KW - ABA

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SN - 0032-0781

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JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 1

ER -

Nitric oxide mediates brassinosteroid-induced ABA biosynthesis involved in oxidative stress tolerance in maize leaves

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