Niaoduqing granules inhibits TGF-β1-induced epithelial-mesenchymal transition in human renal tubular epithelial HK-2 cells

Chu Ying Huo; Hua Yi Yang; Wei Min Ning; Lin Zhong Yu; Chun Lin Fan; Jing Yu Quan; Li Er Deng; Zhi Ling Yu; Jun Shan Liu; Hui Hui Cao

doi:10.53388/MHM2022A0527001

Niaoduqing granules inhibits TGF-β1-induced epithelial-mesenchymal transition in human renal tubular epithelial HK-2 cells

Chu Ying Huo, Hua Yi Yang, Wei Min Ning, Lin Zhong Yu, Chun Lin Fan, Jing Yu Quan, Li Er Deng, Zhi Ling Yu^*, Jun Shan Liu^*, Hui Hui Cao^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

Abstract

Objective: Chronic renal failure (CRF) is a worldwide public health burden. Niaoduqing granules (NDQ) is widely used for CRF treatment in China. However, the underlying mechanism of NDQ is not fully studied. This study is aimed to investigate whether NDQ ameliorate CRF by inhibiting transforming growth factor-β1 (TGF-β1)-induced EMT in human renal tubular epithelial HK-2 cells.

Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay and colony formation assay were used to investigate the cytotoxicity of NDQ in HK-2 cells. Morphological changes of HK-2 cells after TGF-β1 or/and NDQ treatment were observed under a microscope. Wound-healing, migration and invasion assays were performed to determine the cell movement, migratory and invasive abilities, respectively. Western blot analysis was carried out to examine the protein levels of TGF-β receptor I (TβRI) and epithelial-mesenchymal transition (EMT)-associated factors. Fluorescence confocal microscopy was applied to observe the organization of filamentous actin.

Results: NDQ suppressed TβRI expression dose-dependently. NDQ inhibited TGF-β1-stimulated EMT in HK-2 cells, supported by the evidences that NDQ prevented morphology change, attenuated cell migration and invasion, downregulated EMT factors and reorganized filamentous actin distribution in TGF-β1-stimulated HK-2 cells.

Conclusions: NDQ attenuates chronic renal failure which may be associated with inhibition of TβRI expression and EMT process.

Original language	English
Article number	14
Number of pages	9
Journal	TMR Modern Herbal Medicine
Volume	5
Issue number	3
DOIs	https://doi.org/10.53388/MHM2022A0527001
Publication status	Published - 1 Sept 2022

User-Defined Keywords

Chronic renal failure
Niaoduqing granules
TGF-β1
Epithelial-mesenchymal transition
TGF-β type I receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.53388/MHM2022A0527001

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@article{736b85b847ea4f77b663ee301855fb4e,

title = "Niaoduqing granules inhibits TGF-β1-induced epithelial-mesenchymal transition in human renal tubular epithelial HK-2 cells",

abstract = "Objective: Chronic renal failure (CRF) is a worldwide public health burden. Niaoduqing granules (NDQ) is widely used for CRF treatment in China. However, the underlying mechanism of NDQ is not fully studied. This study is aimed to investigate whether NDQ ameliorate CRF by inhibiting transforming growth factor-β1 (TGF-β1)-induced EMT in human renal tubular epithelial HK-2 cells.Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay and colony formation assay were used to investigate the cytotoxicity of NDQ in HK-2 cells. Morphological changes of HK-2 cells after TGF-β1 or/and NDQ treatment were observed under a microscope. Wound-healing, migration and invasion assays were performed to determine the cell movement, migratory and invasive abilities, respectively. Western blot analysis was carried out to examine the protein levels of TGF-β receptor I (TβRI) and epithelial-mesenchymal transition (EMT)-associated factors. Fluorescence confocal microscopy was applied to observe the organization of filamentous actin.Results: NDQ suppressed TβRI expression dose-dependently. NDQ inhibited TGF-β1-stimulated EMT in HK-2 cells, supported by the evidences that NDQ prevented morphology change, attenuated cell migration and invasion, downregulated EMT factors and reorganized filamentous actin distribution in TGF-β1-stimulated HK-2 cells.Conclusions: NDQ attenuates chronic renal failure which may be associated with inhibition of TβRI expression and EMT process.",

keywords = "Chronic renal failure, Niaoduqing granules, TGF-β1, Epithelial-mesenchymal transition, TGF-β type I receptor",

author = "Huo, {Chu Ying} and Yang, {Hua Yi} and Ning, {Wei Min} and Yu, {Lin Zhong} and Fan, {Chun Lin} and Quan, {Jing Yu} and Deng, {Li Er} and Yu, {Zhi Ling} and Liu, {Jun Shan} and Cao, {Hui Hui}",

note = "Funding information: This work was supported by the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (GDHVPS2018), the National Hong Kong Scholars program (XJ2016059), the Natural Science Foundation of Guangdong Province (2020A1515011239), the Guangzhou Science and Technology Project (202102021241), the Administration of Traditional Chinese Medicine of Guangdong Province (20211253) and the Guangzhou Consun Pharmaceuticals Co., Ltd.. These funding bodies played no role in the design of the study; collection, analysis and interpretation of data; and in writing the manuscript. Publisher copyright: {\textcopyright} 2022 By Authors. Published by TMR Publishing Group Limited.",

year = "2022",

month = sep,

day = "1",

doi = "10.53388/MHM2022A0527001",

language = "English",

volume = "5",

journal = "TMR Modern Herbal Medicine",

issn = "2537-9798",

number = "3",

}

TY - JOUR

T1 - Niaoduqing granules inhibits TGF-β1-induced epithelial-mesenchymal transition in human renal tubular epithelial HK-2 cells

AU - Huo, Chu Ying

AU - Yang, Hua Yi

AU - Ning, Wei Min

AU - Yu, Lin Zhong

AU - Fan, Chun Lin

AU - Quan, Jing Yu

AU - Deng, Li Er

AU - Yu, Zhi Ling

AU - Liu, Jun Shan

AU - Cao, Hui Hui

N1 - Funding information: This work was supported by the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (GDHVPS2018), the National Hong Kong Scholars program (XJ2016059), the Natural Science Foundation of Guangdong Province (2020A1515011239), the Guangzhou Science and Technology Project (202102021241), the Administration of Traditional Chinese Medicine of Guangdong Province (20211253) and the Guangzhou Consun Pharmaceuticals Co., Ltd.. These funding bodies played no role in the design of the study; collection, analysis and interpretation of data; and in writing the manuscript. Publisher copyright: © 2022 By Authors. Published by TMR Publishing Group Limited.

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Objective: Chronic renal failure (CRF) is a worldwide public health burden. Niaoduqing granules (NDQ) is widely used for CRF treatment in China. However, the underlying mechanism of NDQ is not fully studied. This study is aimed to investigate whether NDQ ameliorate CRF by inhibiting transforming growth factor-β1 (TGF-β1)-induced EMT in human renal tubular epithelial HK-2 cells.Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay and colony formation assay were used to investigate the cytotoxicity of NDQ in HK-2 cells. Morphological changes of HK-2 cells after TGF-β1 or/and NDQ treatment were observed under a microscope. Wound-healing, migration and invasion assays were performed to determine the cell movement, migratory and invasive abilities, respectively. Western blot analysis was carried out to examine the protein levels of TGF-β receptor I (TβRI) and epithelial-mesenchymal transition (EMT)-associated factors. Fluorescence confocal microscopy was applied to observe the organization of filamentous actin.Results: NDQ suppressed TβRI expression dose-dependently. NDQ inhibited TGF-β1-stimulated EMT in HK-2 cells, supported by the evidences that NDQ prevented morphology change, attenuated cell migration and invasion, downregulated EMT factors and reorganized filamentous actin distribution in TGF-β1-stimulated HK-2 cells.Conclusions: NDQ attenuates chronic renal failure which may be associated with inhibition of TβRI expression and EMT process.

AB - Objective: Chronic renal failure (CRF) is a worldwide public health burden. Niaoduqing granules (NDQ) is widely used for CRF treatment in China. However, the underlying mechanism of NDQ is not fully studied. This study is aimed to investigate whether NDQ ameliorate CRF by inhibiting transforming growth factor-β1 (TGF-β1)-induced EMT in human renal tubular epithelial HK-2 cells.Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay and colony formation assay were used to investigate the cytotoxicity of NDQ in HK-2 cells. Morphological changes of HK-2 cells after TGF-β1 or/and NDQ treatment were observed under a microscope. Wound-healing, migration and invasion assays were performed to determine the cell movement, migratory and invasive abilities, respectively. Western blot analysis was carried out to examine the protein levels of TGF-β receptor I (TβRI) and epithelial-mesenchymal transition (EMT)-associated factors. Fluorescence confocal microscopy was applied to observe the organization of filamentous actin.Results: NDQ suppressed TβRI expression dose-dependently. NDQ inhibited TGF-β1-stimulated EMT in HK-2 cells, supported by the evidences that NDQ prevented morphology change, attenuated cell migration and invasion, downregulated EMT factors and reorganized filamentous actin distribution in TGF-β1-stimulated HK-2 cells.Conclusions: NDQ attenuates chronic renal failure which may be associated with inhibition of TβRI expression and EMT process.

KW - Chronic renal failure

KW - Niaoduqing granules

KW - TGF-β1

KW - Epithelial-mesenchymal transition

KW - TGF-β type I receptor

UR - https://www.tmrjournals.com/article_list.html?J_num=19&y_id=392

U2 - 10.53388/MHM2022A0527001

DO - 10.53388/MHM2022A0527001

M3 - Journal article

SN - 2537-9798

VL - 5

JO - TMR Modern Herbal Medicine

JF - TMR Modern Herbal Medicine

IS - 3

M1 - 14

ER -

Niaoduqing granules inhibits TGF-β1-induced epithelial-mesenchymal transition in human renal tubular epithelial HK-2 cells

Abstract

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