New ribosome-inactivating proteins from seeds and fruits of the bitter gourd Momordica charantia

Paul M.F. Tse, T. B. Ng*, W. P. Fong, Ricky N S WONG, C. C. Wan, Nai Ki MAK, H. W. Yeung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A new ribosome-inactivating protein (RIP) (δ-momorcharin) and a candidate RIP (ε-momorcharin) were isolated, respectively, from the seeds and fruits of the bitter gourd Momordica charantia, by affinity chromatography on Affi-gel blue gel and ion exchange chromatography on Mono S with a fast protein liquid chromatography (FPLC) system. Both δ- and ε-momorcharins were adsorbed on Affi-gel blue gel and were eluted from the cation exchange Mono S column earlier than α- and β-momorcharins, the two previously reported RIPs, δ- and ε-momorcharins possessed a molecular weight of 30 and 24 kDa respectively and inhibited cell-free translation in rabbit reticulocyte lysate with an IC50 of 0.15 and 170 nM. The sequence of the first seven N-terminal amino acids in δ-momorcharin was DVNFGLA, which was different from the N-terminal sequences DVSFRLS and DVNFDLS for α- and β-momorcharins respectively. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)895-901
Number of pages7
JournalInternational Journal of Biochemistry and Cell Biology
Volume31
Issue number9
DOIs
Publication statusPublished - Sep 1999

Scopus Subject Areas

  • Biochemistry
  • Cell Biology

User-Defined Keywords

  • Bitter gourd
  • Momorcharins
  • Ribosome-inactivating protein

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