Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells

Xiao Fan Li; Cathy N. Lui; Zhi Hong JIANG; Kin Lam YUNG

doi:10.1186/1749-8546-6-19

Neuroprotective effects of ginsenosides Rh₁and Rg₂on neuronal cells

Xiao Fan Li, Cathy N. Lui, Zhi Hong JIANG, Kin Lam YUNG^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

18 Citations (Scopus)

Abstract

Background: The present study investigates the effects of ginsenosides Rh₁and Rg₂against 6-hydroxydopamine (6-OHDA), a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined.Methods: LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides' effects on activation of extracellular signal-regulated protein kinases (ERKs).Results: Rh₁and Rg₂attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh₁and Rg₂. 20(R)-form and 20(S)-form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells.Conclusion: The present study demonstrates neuroprotective effects of ginsenosides Rh₁and Rg₂on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders (eg Parkinson's disease).

Original language	English
Article number	19
Number of pages	9
Journal	Chinese Medicine
Volume	6
DOIs	https://doi.org/10.1186/1749-8546-6-19
Publication status	Published - 19 May 2011

Scopus Subject Areas

Pharmacology
Complementary and alternative medicine

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title = "Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells",

abstract = "Background: The present study investigates the effects of ginsenosides Rh1 and Rg2 against 6-hydroxydopamine (6-OHDA), a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined.Methods: LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides' effects on activation of extracellular signal-regulated protein kinases (ERKs).Results: Rh1 and Rg2 attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh1 and Rg2. 20(R)-form and 20(S)-form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells.Conclusion: The present study demonstrates neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders (eg Parkinson's disease).",

author = "Li, {Xiao Fan} and Lui, {Cathy N.} and JIANG, {Zhi Hong} and YUNG, {Kin Lam}",

year = "2011",

month = may,

day = "19",

doi = "10.1186/1749-8546-6-19",

language = "English",

volume = "6",

journal = "Chinese Medicine",

issn = "1749-8546",

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TY - JOUR

T1 - Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells

AU - Li, Xiao Fan

AU - Lui, Cathy N.

AU - JIANG, Zhi Hong

AU - YUNG, Kin Lam

PY - 2011/5/19

Y1 - 2011/5/19

N2 - Background: The present study investigates the effects of ginsenosides Rh1 and Rg2 against 6-hydroxydopamine (6-OHDA), a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined.Methods: LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides' effects on activation of extracellular signal-regulated protein kinases (ERKs).Results: Rh1 and Rg2 attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh1 and Rg2. 20(R)-form and 20(S)-form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells.Conclusion: The present study demonstrates neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders (eg Parkinson's disease).

AB - Background: The present study investigates the effects of ginsenosides Rh1 and Rg2 against 6-hydroxydopamine (6-OHDA), a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined.Methods: LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides' effects on activation of extracellular signal-regulated protein kinases (ERKs).Results: Rh1 and Rg2 attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh1 and Rg2. 20(R)-form and 20(S)-form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells.Conclusion: The present study demonstrates neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders (eg Parkinson's disease).

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U2 - 10.1186/1749-8546-6-19

DO - 10.1186/1749-8546-6-19

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SN - 1749-8546

VL - 6

JO - Chinese Medicine

JF - Chinese Medicine

M1 - 19

ER -

Neuroprotective effects of ginsenosides Rh₁and Rg₂on neuronal cells

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