Neuroprotective effect of luteolin on amyloid β protein (25-35)-induced toxicity in cultured rat cortical neurons

Hao Yuan Cheng, Ming Tsuen Hsieh, Fan Shiu Tsai, Chi Rei Wu, Chuan Sung Chiu, Min Min Lee, Hong Xi Xu, Zhongzhen ZHAO, Wen Huang Peng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

The present study was carried out to investigate the neuroprotective effect of luteolin on amyloid β (Aβ) (25-35)-induced neurotoxicity using cultured rat cortical neurons. After exposure of primary cultures of rat cortical cells to 10 μM Aβ (25-35) for 48 h, cortical cell cultures exhibited marked apoptotic death. Pretreatment with luteolin (1, 10 μM) significantly protected cortical cell cultures against Aβ (25-35)-induced toxicity. Luteolin (1, 10 μM) showed a concentration-dependent inhibition on 10 μM Aβ (25-35)-induced apoptotic neuronal death, as assessed by MTT assay. Furthermore, luteolin reduced apoptotic characteristics by DAPI staining. For Western blot analysis, the results showed that the protective effect of luteolin on Aβ (25-35)-induced neurotoxicity was mediated by preventing of ERK-p, JNK, JNK-p, P38-p and caspase 3 activations in rat primary cortical cultures. Taken together, the results suggest that luteolin prevents Aβ (25-35)-induced apoptotic neuronal death through inhibiting the protein level of JNK, ERK and p38 MAP kinases and caspase 3 activations.

Original languageEnglish
Pages (from-to)S102-S108
JournalPhytotherapy Research
Volume24
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jan 2010

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Caspase 3
  • Cortical neurons
  • Luteolin
  • MAP kinases

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