Near-infrared luminescence quenching method for the detection of phenolic compounds using N-acetyl-l-cysteine-protected gold nanoparticles-tyrosinase hybrid material

Wenjuan Dong, Chuan Dong*, Shaomin Shuang, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A rapid and simple near-infrared (NIR) luminescence quenching method for the detection of phenolic compounds based on combining the unique property of N-acetyl-l-cysteine-protected gold nanoparticles (NAC-AuNPs) and tyrosinase (Tyr) enzymatic reactions is described. This method relies on the luminescence quenching of NAC-AuNPs-tyrosinase (NAC-AuNPs-Tyr) hybrid material by phenolic compounds. The quinone intermediates produced from enzymatic catalytic oxidation of phenolic compounds were believed to play a major role in the luminescence quenching. Dynamic quenching mechanism was confirmed by using time-resolved luminescence spectroscopy. Optimization of the experimental parameters including the concentration of NAC-AuNPs-Tyr (20 μg/mL), excitation wavelength (450 nm), pH (6.0), and temperature (20 °C) has been determined. A linear range 0.5 μM to 1.0 mM and a detection limit 0.1 μM of catechol were obtained under optimal conditions. The sensitivity of different phenolic compounds was compared and follows the trend: catechol > p-cresol > phenol. The proposed NIR luminescence quenching method exhibits high sensitivity, good repeatability, and long-term stability, demonstrating potential for further development to NIR luminescence phenol biosensors.

Original languageEnglish
Pages (from-to)1043-1048
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number5
DOIs
Publication statusPublished - 15 Jan 2010

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Gold nanoparticles
  • Near-infrared fluorescence
  • Phenolic compounds
  • Tyrosinase

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