Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies

Neng Hui Ye, Feng Zhu Wang, Lu Shi, Mo Xian Chen, Yun Ying Cao, Fu Yuan Zhu, Yi Zhen Wu, Li Juan Xie, Tie Yuan Liu, Ze Zhuo Su, Shi Xiao, Hao Zhang, Jianchang Yang, Hai Yong Gu, Xuan Xuan Hou, Qi Juan Hu, Hui Juan Yi, Chang Xiang Zhu, Jianhua ZHANG*, Ying Gao Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress. In this study, two rice cultivars, upland (Up221, flooding-intolerant) and lowland (Low88, flooding-tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding-tolerant type (T-type) or a flooding-intolerant type (I-type). The OsCBL10 T-type promoter only existed in japonica lowland cultivars, whereas the OsCBL10 I-type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding-tolerant rice cultivars containing the OsCBL10 T-type promoter have shown lower Ca2+ flow and higher α-amylase activities in comparison to those in flooding-intolerant cultivars. Furthermore, the OsCBL10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca2+ flow in comparison to wild-type. Subsequent findings also indicate that OsCBL10 may affect OsCIPK15 protein abundance and its downstream pathways. In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different OsCBL10 promoters, which in turn affect OsCBL10 expression in different cultivars and negatively affect OsCIPK15 protein accumulation and its downstream cascade.

Original languageEnglish
Pages (from-to)612-625
Number of pages14
JournalPlant Journal
Volume94
Issue number4
DOIs
Publication statusPublished - May 2018

Scopus Subject Areas

  • Genetics
  • Plant Science
  • Cell Biology

User-Defined Keywords

  • calcium signaling
  • flooding tolerance
  • hypoxia stress
  • natural variation
  • Oryza sativa
  • seed germination

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