Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Zhihe Liu, Jie Liu, Zhe Zhang, Zezhou Sun, Xuyang Shao, Jintong Guo, Lei Xi, Zhen Yuan, Xuanjun Zhang, Daniel T. Chiu, Changfeng Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

Original languageEnglish
Pages (from-to)7522-7526
Number of pages5
JournalNanoscale
Volume12
Issue number14
Early online date18 Mar 2020
DOIs
Publication statusPublished - 14 Apr 2020

Scopus Subject Areas

  • Materials Science(all)

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