Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Zhihe Liu; Jie Liu; Zhe Zhang; Zezhou Sun; Xuyang Shao; Jintong Guo; Lei Xi; Zhen Yuan; Xuanjun Zhang; Daniel T. Chiu; Changfeng Wu

doi:10.1039/d0nr00347f

Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Zhihe Liu, Jie Liu, Zhe Zhang, Zezhou Sun, Xuyang Shao, Jintong Guo, Lei Xi, Zhen Yuan, Xuanjun Zhang, Daniel T. Chiu, Changfeng Wu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

16 Citations (Scopus)

Abstract

Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8^th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8^th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

Original language	English
Pages (from-to)	7522-7526
Number of pages	5
Journal	Nanoscale
Volume	12
Issue number	14
Early online date	18 Mar 2020
DOIs	https://doi.org/10.1039/d0nr00347f
Publication status	Published - 14 Apr 2020

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Materials Science(all)

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title = "Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging",

abstract = "Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.",

author = "Zhihe Liu and Jie Liu and Zhe Zhang and Zezhou Sun and Xuyang Shao and Jintong Guo and Lei Xi and Zhen Yuan and Xuanjun Zhang and Chiu, {Daniel T.} and Changfeng Wu",

note = "Funding Information: This work was financially supported by the grants from Shenzhen Science and Technology Innovation Commission (Grant No. KQTD20170810111314625), the National Natural Science Foundation of China (Grant No. 81771930). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry .",

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language = "English",

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T1 - Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

AU - Liu, Zhihe

AU - Liu, Jie

AU - Zhang, Zhe

AU - Sun, Zezhou

AU - Shao, Xuyang

AU - Guo, Jintong

AU - Xi, Lei

AU - Yuan, Zhen

AU - Zhang, Xuanjun

AU - Chiu, Daniel T.

AU - Wu, Changfeng

N1 - Funding Information: This work was financially supported by the grants from Shenzhen Science and Technology Innovation Commission (Grant No. KQTD20170810111314625), the National Natural Science Foundation of China (Grant No. 81771930). Publisher Copyright: © The Royal Society of Chemistry .

PY - 2020/4/14

Y1 - 2020/4/14

N2 - Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

AB - Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

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Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

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