Nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms

Chaminda Jayampath Seneviratne, Ken C F LEUNG, Chi Hin Wong, Siu Fung Lee, Xuan Li, Ping Chung Leung, Clara Bik San Lau, Elaine Wat, Lijian Jin

Research output: Contribution to journalJournal articlepeer-review

66 Citations (Scopus)


Background: Chlorhexidine (CHX) is a widely used antimicrobial agent in dentistry. Herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure CHX (Nano-CHX), and its mechanical profile and antimicrobial properties against oral biofilms. Methodology/Principal Findings: The release of CHX from the Nano-CHX was characterized by UV/visible absorption spectroscopy. The antimicrobial properties of Nano-CHX were evaluated in both planktonic and biofilm modes of representative oral pathogenic bacteria. The Nano-CHX demonstrated potent antibacterial effects on planktonic bacteria and mono-species biofilms at the concentrations of 50-200 μg/mL against Streptococcus mutans , Streptococcus sobrinus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Enterococccus faecalis. Moreover, Nano-CHX effectively suppressed multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans and Porphyromonas gingivalis up to 72 h. Conclusions/Significance: This pioneering study demonstrates the potent antibacterial effects of the Nano-CHX on oral biofilms, and it may be developed as a novel and promising anti-biofilm agent for clinical use.

Original languageEnglish
Article numbere103234
JournalPLoS ONE
Issue number8
Publication statusPublished - 29 Aug 2014

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