Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging

Ken Cham Fai Leung; Siu Fung Lee; Chi Hin Wong; Chun Pong Chak; Josie M.Y. Lai; Xiao Ming Zhu; Yi Xiang J. Wang; Kathy W.Y. Sham; Christopher H.K. Cheng

doi:10.1016/j.ymeth.2013.06.006

Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging

Ken Cham Fai Leung^*
, Siu Fung Lee
, Chi Hin Wong
, Chun Pong Chak
, Josie M.Y. Lai
, Xiao Ming Zhu
, Yi Xiang J. Wang
, Kathy W.Y. Sham
, Christopher H.K. Cheng

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.

Original language	English
Pages (from-to)	315-321
Number of pages	7
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.06.006
Publication status	Published - 15 Dec 2013

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Cellular uptake
Gene delivery
Magnetic resonance imaging
Magnetofection
Nanoparticle
Self-assembly

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10.1016/j.ymeth.2013.06.006

Cite this

@article{b81423fd379c456583b1a3bfde24f3cd,

title = "Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging",

abstract = "This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.",

keywords = "Cellular uptake, Gene delivery, Magnetic resonance imaging, Magnetofection, Nanoparticle, Self-assembly",

author = "Leung, \{Ken Cham Fai\} and Lee, \{Siu Fung\} and Wong, \{Chi Hin\} and Chak, \{Chun Pong\} and Lai, \{Josie M.Y.\} and Zhu, \{Xiao Ming\} and Wang, \{Yi Xiang J.\} and Sham, \{Kathy W.Y.\} and Cheng, \{Christopher H.K.\}",

note = "Funding Information: We acknowledge the financial support by HKRGC-GRF ( 201412 ), HKBU-FRG ( FRG1/11-12/031 ), and AoE/P-03/08 research grants.",

year = "2013",

month = dec,

day = "15",

doi = "10.1016/j.ymeth.2013.06.006",

language = "English",

volume = "64",

pages = "315--321",

journal = "Methods",

issn = "1046-2023",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging

AU - Leung, Ken Cham Fai

AU - Lee, Siu Fung

AU - Wong, Chi Hin

AU - Chak, Chun Pong

AU - Lai, Josie M.Y.

AU - Zhu, Xiao Ming

AU - Wang, Yi Xiang J.

AU - Sham, Kathy W.Y.

AU - Cheng, Christopher H.K.

N1 - Funding Information: We acknowledge the financial support by HKRGC-GRF ( 201412 ), HKBU-FRG ( FRG1/11-12/031 ), and AoE/P-03/08 research grants.

PY - 2013/12/15

Y1 - 2013/12/15

N2 - This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.

AB - This paper describes comparative studies and protocols in (1) self-assembling of ultrasmall superparamagnetic iron oxide nanoparticle (NP), circular plasmid DNA, and branched polyethylenimine (PEI) composites; (2) magnetofection; (3) gene delivery, (4) magnetic resonance imaging (MRI), and (5) cytotoxicity of the composites toward hepatocellular carcinoma HepG2 cells.

KW - Cellular uptake

KW - Gene delivery

KW - Magnetic resonance imaging

KW - Magnetofection

KW - Nanoparticle

KW - Self-assembly

UR - https://www.scopus.com/pages/publications/84888219306

U2 - 10.1016/j.ymeth.2013.06.006

DO - 10.1016/j.ymeth.2013.06.006

M3 - Journal article

C2 - 23811300

AN - SCOPUS:84888219306

SN - 1046-2023

VL - 64

SP - 315

EP - 321

JO - Methods

JF - Methods

IS - 3

ER -

Nanoparticle-DNA-polymer composites for hepatocellular carcinoma cell labeling, sensing, and magnetic resonance imaging

Abstract

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