N-WASP Is Required for Structural Integrity of the Blood-Testis Barrier

Xiang Xiao, Dolores D. Mruk, Elizabeth I. Tang, R'ada Massarwa, Ka Wai Mok, Nan Li, Chris K C WONG, Will M. Lee, Scott B. Snapper, Ben Zion Shilo, Eyal D. Schejter, C. Yan Cheng

Research output: Contribution to journalJournal articlepeer-review

28 Citations (Scopus)


During spermatogenesis, the blood-testis barrier (BTB) segregates the adluminal (apical) and basal compartments in the seminiferous epithelium, thereby creating a privileged adluminal environment that allows post-meiotic spermatid development to proceed without interference of the host immune system. A key feature of the BTB is its continuous remodeling within the Sertoli cells, the major somatic component of the seminiferous epithelium. This remodeling is necessary to allow the transport of germ cells towards the seminiferous tubule interior, while maintaining intact barrier properties. Here we demonstrate that the actin nucleation promoting factor Neuronal Wiskott-Aldrich Syndrome Protein (N-WASP) provides an essential function necessary for BTB restructuring, and for maintaining spermatogenesis. Our data suggests that the N-WASP-Arp2/3 actin polymerization machinery generates branched-actin arrays at an advanced stage of BTB remodeling. These arrays are proposed to mediate the restructuring process through endocytic recycling of BTB components. Disruption of N-WASP in Sertoli cells results in major structural abnormalities to the BTB, including mis-localization of critical junctional and cytoskeletal elements, and leads to disruption of barrier function. These impairments result in a complete arrest of spermatogenesis, underscoring the critical involvement of the somatic compartment of the seminiferous tubules in germ cell maturation.

Original languageEnglish
Article numbere1004447
JournalPLoS Genetics
Issue number6
Publication statusPublished - Jun 2014

Scopus Subject Areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research


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