Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China

Mengsu Yang; Hong Zhou; Richard Y.C. Kong; David W F Fong; Li Qiang Ren; Xiang Hai Liao; Yufang Wang; Weijian Zhuang; Shanming Yang

doi:10.1016/S0027-5107(97)00142-5

Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China

Mengsu Yang^*, Hong Zhou, Richard Y.C. Kong, David W F Fong, Li Qiang Ren, Xiang Hai Liao, Yufang Wang, Weijian Zhuang, Shanming Yang

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

25 Citations (Scopus)

Abstract

Codon 249, (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100 000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G → T transversion is positively associated with the level of dietary aflatoxin B₁ (AFB₁) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 249 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.

Original language	English
Pages (from-to)	25-29
Number of pages	5
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	381
Issue number	1
DOIs	https://doi.org/10.1016/S0027-5107(97)00142-5
Publication status	Published - 19 Nov 1997

Scopus Subject Areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

User-Defined Keywords

Codon 249 mutation
Hepatocellular carcinoma
p53 gene

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10.1016/S0027-5107(97)00142-5

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title = "Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China",

abstract = "Codon 249, (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100 000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G → T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 249 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.",

keywords = "Codon 249 mutation, Hepatocellular carcinoma, p53 gene",

author = "Mengsu Yang and Hong Zhou and Kong, {Richard Y.C.} and Fong, {David W F} and Ren, {Li Qiang} and Liao, {Xiang Hai} and Yufang Wang and Weijian Zhuang and Shanming Yang",

note = "Funding Information: This work is supported by the Industry Support Fund, Hong Kong Government Industry Department, and the City University of Hong Kong. Technical assistance and comments from Professor F. Yang of the Third Institute of SOA in China is gratefully acknowledged.",

year = "1997",

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doi = "10.1016/S0027-5107(97)00142-5",

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AU - Yang, Mengsu

AU - Zhou, Hong

AU - Kong, Richard Y.C.

AU - Fong, David W F

AU - Ren, Li Qiang

AU - Liao, Xiang Hai

AU - Wang, Yufang

AU - Zhuang, Weijian

AU - Yang, Shanming

N1 - Funding Information: This work is supported by the Industry Support Fund, Hong Kong Government Industry Department, and the City University of Hong Kong. Technical assistance and comments from Professor F. Yang of the Third Institute of SOA in China is gratefully acknowledged.

PY - 1997/11/19

Y1 - 1997/11/19

N2 - Codon 249, (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100 000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G → T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 249 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.

AB - Codon 249, (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100 000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G → T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 249 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.

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Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China

Abstract

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