Mutations at codon 249 of p53 gene in human hepatocellular carcinomas from Tongan, China

Mengsu Yang*, Hong Zhou, Richard Y.C. Kong, David W F FONG, Li Qiang Ren, Xiang Hai Liao, Yufang Wang, Weijian Zhuang, Shanming Yang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Codon 249, (exon 7) of the putative tumor suppressor gene p53 is a mutational hot-spot for hepatocellular carcinoma (HCC) but not other tumors. DNA samples from primary HCC patients from Tongan, an area of high HCC incidence in China (> 40 per 100 000 population), were analyzed for specific mutations in codon 249 of the p53 gene using polymerase chain reaction (PCR)/restriction-digest methods and direct DNA sequencing. Seven of the 21 samples screened were found to have a point mutation at the third base position of codon 249 (AGG to AGT). The result is consistent with previous reports that the G → T transversion is positively associated with the level of dietary aflatoxin B1 (AFB1) contamination, which has been implicated as one of the risk factors in Tongan area. Of the 249 HCC patients that contained the codon 249 point mutation, one was hepatitis B virus (HBV)-negative. This is only the second documentation of an HCC patient harboring the p53 codon 249 mutation, who was HBV-negative.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume381
Issue number1
DOIs
Publication statusPublished - 19 Nov 1997

Scopus Subject Areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

User-Defined Keywords

  • Codon 249 mutation
  • Hepatocellular carcinoma
  • p53 gene

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