TY - JOUR
T1 - Multiple organ injury in male C57BL/6J mice exposed to ambient particulate matter in a real-ambient PM exposure system in Shijiazhuang, China
AU - Li, Daochuan
AU - Zhang, Rong
AU - Cui, Lianhua
AU - Chu, Chen
AU - Zhang, Haiyan
AU - Sun, Hao
AU - Luo, Jing
AU - Zhou, Lixiao
AU - Chen, Liping
AU - Cui, Jian
AU - Chen, Shen
AU - Mai, Bixian
AU - Chen, Shejun
AU - Yu, Jianzhen
AU - CAI, Zongwei
AU - Zhang, Jianqing
AU - Jiang, Yousheng
AU - Aschner, Michael
AU - Chen, Rui
AU - Zheng, Yuxin
AU - Chen, Wen
N1 - Funding Information:
We thank Dr. Li Liu (Pathologist of Mercy Medical Center, MD, USA) for confirmation of pathological diagnosis), and Dr. Hongbao Yang for technical support. This work was supported by the Major Research Plan of National Natural Science Foundation of China (key grants: 91543208, 91643203, training grants: 91643109, 91643108, 91543130), the State Key Program of National Natural Science Foundation of China (81730088), Guangdong Provincial Natural Science Foundation Team Project (2018B030312005) and National Institute of Environmental Health Sciences of USA (R01 ES10563, R01 ES07331 and R01 ES020852).
Funding Information:
We thank Dr. Li Liu (Pathologist of Mercy Medical Center, MD, USA) for confirmation of pathological diagnosis), and Dr. Hongbao Yang for technical support. This work was supported by the Major Research Plan of National Natural Science Foundation of China (key grants: 91543208 , 91643203 , training grants: 91643109 , 91643108 , 91543130 ), the State Key Program of National Natural Science Foundation of China ( 81730088 ), Guangdong Provincial Natural Science Foundation Team Project ( 2018B030312005 ) and National Institute of Environmental Health Sciences of USA ( R01 ES10563 , R01 ES07331 and R01 ES020852 ).
PY - 2019/5
Y1 - 2019/5
N2 - The development of a rodent ambient particulate matter (PM) inhalation system is critical for drawing causal inferences between PM exposure and the onset of human diseases. In this study, we constructed a real-ambient PM exposure system to investigate multi-organ injury and the reversibility of the impairments in C57BL/6 J male mice exposed to PM with a duration of up to three months in Shijiazhuang, a city with the highest PM2.5 concentration in China. This unique exposure system provided an optimal scenario for round-the-clock PM exposure absent a change in the physiochemical properties of PM and minimized the disturbance to the mice habitat. The mean concentration of PM2.5 in the exposure chambers was 89.95, 79.98, and 87.87 μg/m3 at three different time points, respectively: weeks 1–3, week 1–6, and week 1–12. The injury in multiple organs, including lung, brain, heart, testis, and intestine, was profound and was evident by the significant pathological and functional alterations. Pulmonary pathological examination revealed severe interstitial inflammatory and alveolar hemorrhage throughout the exposure, which was in line with the reduced lung function and the increased cytokine excretion in bronchoalveolar lavage fluid and blood plasma. Notably, the PM-mediated inflammatory response in different systems was correlated with the severity of the injury and the attenuation of pulmonary lesions in the recovery group. Thus, the PM2.5-induced inflammatory response, the chemical components-induced cytotoxicity, genetic damage, and oxidative stress might be implicated in the impairment of multiple murine organs. These findings revealed the severity, sensitivity, and reversibility of multi-organ injury in response to a real-ambient PM exposure.
AB - The development of a rodent ambient particulate matter (PM) inhalation system is critical for drawing causal inferences between PM exposure and the onset of human diseases. In this study, we constructed a real-ambient PM exposure system to investigate multi-organ injury and the reversibility of the impairments in C57BL/6 J male mice exposed to PM with a duration of up to three months in Shijiazhuang, a city with the highest PM2.5 concentration in China. This unique exposure system provided an optimal scenario for round-the-clock PM exposure absent a change in the physiochemical properties of PM and minimized the disturbance to the mice habitat. The mean concentration of PM2.5 in the exposure chambers was 89.95, 79.98, and 87.87 μg/m3 at three different time points, respectively: weeks 1–3, week 1–6, and week 1–12. The injury in multiple organs, including lung, brain, heart, testis, and intestine, was profound and was evident by the significant pathological and functional alterations. Pulmonary pathological examination revealed severe interstitial inflammatory and alveolar hemorrhage throughout the exposure, which was in line with the reduced lung function and the increased cytokine excretion in bronchoalveolar lavage fluid and blood plasma. Notably, the PM-mediated inflammatory response in different systems was correlated with the severity of the injury and the attenuation of pulmonary lesions in the recovery group. Thus, the PM2.5-induced inflammatory response, the chemical components-induced cytotoxicity, genetic damage, and oxidative stress might be implicated in the impairment of multiple murine organs. These findings revealed the severity, sensitivity, and reversibility of multi-organ injury in response to a real-ambient PM exposure.
KW - Inflammation
KW - Mouse model
KW - Multi-organ injury
KW - Particulate matter
KW - Real-ambient PM exposure system
UR - http://www.scopus.com/inward/record.url?scp=85062514092&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2019.02.097
DO - 10.1016/j.envpol.2019.02.097
M3 - Journal article
C2 - 30856503
AN - SCOPUS:85062514092
SN - 0269-7491
VL - 248
SP - 874
EP - 887
JO - Environmental Pollution
JF - Environmental Pollution
ER -
