TY - JOUR
T1 - Multicolor Immunodiagnostic for Semiquantitative Visual Detection Syndrome Coronavirus 2 Specific Antibody
T2 - A Prospect Strategy for On-site COVID-19 Therapeutic Process Monitoring
AU - Chen, Zhonghui
AU - Li, Jinqiu
AU - Lin, Wei
AU - Yu, Liumin
AU - Tu, Haijian
AU - Chen, Yu
AU - Cai, Zongwei
AU - Lin, Zhenyu
N1 - Publisher Copyright:
© 2021, Editorial Department of Chem. J. Chinese Universities. All right reserved.
PY - 2021/11/10
Y1 - 2021/11/10
N2 - Rapid detection of body fluid severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) antibody is an effective strategy for infection therapeutic effect of coronavirus disease(COVID-19). Most detection methods require relatively large equipment, which limited their on-site application. Lateral flow immunoassay(LFIA) can be used to qualitative antibody detection based on the aggregation of gold nanoparticles (Au NPs), which exhibits just one-color change and cannot realize rapid quantitative detection without the help of additional equipment. In this study, a high-resolution multicolor colorimetric strategy was developed and applied to assessing antibody concentration at a glance based on etching of gold nanorods(Au NRs). Firstly, SARS-CoV-2 recombinant antigen was immobilized on the surface of the 96-wells. Then, horseradish peroxidase(HRP)-labeled second antibody combined with antibody to form an antigen-antibody-secondary antibody complex on the well surface, which has direct relationship with antibody concentration in the sample and can be used to oxidize 3, 3', 5, 5'-tetramethylbenzidine(TMB) to form TMB2+ at the presence of HRP. The generation of TMB2+ efficiently etch Au NRs to produce multicolor solution. The etching result in vivid color changes in the system has a relationship with the amount of SARS-CoV-2 IgM antibody. Under the optimal conditions, the proposed strategy exhibited a linear response in the 5.00―200 IU concentration range, and a detection limit of 1.29 IU for SARS-CoV-2 IgM antibody, with high sensitivity and specificity. This assay is prospective for the on-site semi-quantitative visual detection of SARS-CoV-2 IgM antibody concentration in the COVID-19 therapeutic process.
AB - Rapid detection of body fluid severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) antibody is an effective strategy for infection therapeutic effect of coronavirus disease(COVID-19). Most detection methods require relatively large equipment, which limited their on-site application. Lateral flow immunoassay(LFIA) can be used to qualitative antibody detection based on the aggregation of gold nanoparticles (Au NPs), which exhibits just one-color change and cannot realize rapid quantitative detection without the help of additional equipment. In this study, a high-resolution multicolor colorimetric strategy was developed and applied to assessing antibody concentration at a glance based on etching of gold nanorods(Au NRs). Firstly, SARS-CoV-2 recombinant antigen was immobilized on the surface of the 96-wells. Then, horseradish peroxidase(HRP)-labeled second antibody combined with antibody to form an antigen-antibody-secondary antibody complex on the well surface, which has direct relationship with antibody concentration in the sample and can be used to oxidize 3, 3', 5, 5'-tetramethylbenzidine(TMB) to form TMB2+ at the presence of HRP. The generation of TMB2+ efficiently etch Au NRs to produce multicolor solution. The etching result in vivid color changes in the system has a relationship with the amount of SARS-CoV-2 IgM antibody. Under the optimal conditions, the proposed strategy exhibited a linear response in the 5.00―200 IU concentration range, and a detection limit of 1.29 IU for SARS-CoV-2 IgM antibody, with high sensitivity and specificity. This assay is prospective for the on-site semi-quantitative visual detection of SARS-CoV-2 IgM antibody concentration in the COVID-19 therapeutic process.
KW - Antibody
KW - Multicolor colorime-tric strategy
KW - Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
KW - Therapeutic process monitoring
UR - http://www.scopus.com/inward/record.url?scp=85118923766&partnerID=8YFLogxK
U2 - 10.7503/cjcu20210363
DO - 10.7503/cjcu20210363
M3 - Journal article
AN - SCOPUS:85118923766
SN - 0251-0790
VL - 42
SP - 3509
EP - 3518
JO - Chemical Journal of Chinese Universities
JF - Chemical Journal of Chinese Universities
IS - 11
ER -