@article{893a88fc13894eaeaeaa3e83d35acee3,
title = "m5C Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants",
abstract = "In plants, thousands of messenger RNAs move to distant body parts to potentially act as systemic signals. Yang, Pererra, et al. show that 5-methylcytosine (m5C) modification of mobile RNA plays a crucial role in facilitating their transport and present evidence that mobile m5C-modified TCTP1 is translated in target cells and changes root growth.",
keywords = "grafting, long-distance transport, mC, mRNA methylation, mRNA modification, mRNA transport, phloem, plasmodesmata, root development",
author = "Lei Yang and Valentina Perrera and Eleftheria Saplaoura and Federico Apelt and Mathieu Bahin and Amira Kramdi and Justyna Olas and Bernd Mueller-Roeber and Ewelina Sokolowska and Wenna Zhang and Runsheng LI and Nicolas Pitzalis and Manfred Heinlein and Shoudong ZHANG and Auguste Genovesio and Vincent Colot and Friedrich Kragler",
note = "Funding Information: We thank Dana Schindelasch (MPI-MPP), Marina Stratmann (MPI-MPP), and Eike Kamann (University of Potsdam) for their excellent technical support and Frank Marchin (MPI-MPP) for comments on the manuscript. V.P. thanks Thomas Jenuwein for his support at MPI-IE Freiburg, where she established the MeRIP protocol used in the study. We thank Iain Robert Searle (University of Adelaide) for providing m5C methylation-deficient mutant seeds and Marcin Janowski and Marek Mutwil (MPI-MPP) for the UBQ10::HSC70.1-GFP lines. This work was supported by Investissements d'Avenir ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001-02 PSL? Research University to V.C. DFG project A5, CRC 973 to B.M.-R. MPI-MPP internal funds to F.K. a Chinese Scholarship Council PhD stipend to L.Y. an EMBO Long-Term Fellowship to V.P. and an IMPRS PhD stipend to E.S. Part of the project was supported by ERC-Syg PLAMORF grant money awarded to F.K. V.P. S.Z. V.C. and F.K. conceived the idea of the project; L.Y. F.K. V.P. E. Saplaoura, N.P. and S.Z. performed experiments; L.Y. produced transgenic plants, performed all grafting and growth experiments, and with W.Z. produced TCTP1 deletion constructs; E. Sokolowska produced the ethanol-inducible transgenic plants; F.A. E. Saplaoura, M.B. A.K. and A.G. analyzed the RNA-seq data; B.M.-R. and J.O. provided infrastructure and suggested experiments performed by J.O.; M.H. suggested experiments and supervised experiments performed by N.P.; F.K. with L.Y. E. Saplaoura, V.P. and F.A. wrote the manuscript with input from V.C. M.H. and B.M.-R. All authors read the manuscript and expressed their consent to submit the final version of the manuscript. The authors declare no competing interests. Funding Information: We thank Dana Schindelasch (MPI-MPP), Marina Stratmann (MPI-MPP), and Eike Kamann (University of Potsdam) for their excellent technical support and Frank Marchin (MPI-MPP) for comments on the manuscript. V.P. thanks Thomas Jenuwein for his support at MPI-IE Freiburg, where she established the MeRIP protocol used in the study. We thank Iain Robert Searle (University of Adelaide) for providing m 5 C methylation-deficient mutant seeds and Marcin Janowski and Marek Mutwil (MPI-MPP) for the UBQ10::HSC70.1-GFP lines. This work was supported by Investissements d{\textquoteright}Avenir ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001-02 PSL ∗ Research University to V.C., DFG project A5, CRC 973 to B.M.-R., MPI-MPP internal funds to F.K., a Chinese Scholarship Council PhD stipend to L.Y., an EMBO Long-Term Fellowship to V.P., and an IMPRS PhD stipend to E.S. Part of the project was supported by ERC-Syg PLAMORF grant money awarded to F.K. ",
year = "2019",
month = aug,
day = "5",
doi = "10.1016/j.cub.2019.06.042",
language = "English",
volume = "29",
pages = "2465--2476.e5",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "15",
}