Monolithic integration of fine cylindrical glass microcapillaries on silicon for electrophoretic separation of biomolecules

Zhen Cao; Kangning REN; Hongkai Wu; Levent Yobas

doi:10.1063/1.4739075

Monolithic integration of fine cylindrical glass microcapillaries on silicon for electrophoretic separation of biomolecules

Zhen Cao, Kangning REN, Hongkai Wu, Levent Yobas^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

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Abstract

We demonstrate monolithic integration of fine cylindrical glass microcapillaries (diameter ~1 μm) on silicon and evaluate their performance for electrophoretic separation of biomolecules. Such microcapillaries are achieved through thermal reflow of a glass layer on microstructured silicon whereby slender voids are moulded into cylindrical tubes. The process allows self-enclosed microcapillaries with a uniform profile. A simplified method is also described to integrate the microcapillaries with a sample-injection cross without the requirement of glass etching. The 10-mm-long microcapillaries sustain field intensities up to 90 kV/m and limit the temperature excursions due to Joule heating to a few degrees Celsius only.

Original language	English
Article number	036501
Journal	Biomicrofluidics
Volume	6
Issue number	3
DOIs	https://doi.org/10.1063/1.4739075
Publication status	Published - 18 Jul 2012

Scopus Subject Areas

Molecular Biology
Materials Science(all)
Genetics
Condensed Matter Physics
Physical and Theoretical Chemistry

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abstract = "We demonstrate monolithic integration of fine cylindrical glass microcapillaries (diameter ~1 μm) on silicon and evaluate their performance for electrophoretic separation of biomolecules. Such microcapillaries are achieved through thermal reflow of a glass layer on microstructured silicon whereby slender voids are moulded into cylindrical tubes. The process allows self-enclosed microcapillaries with a uniform profile. A simplified method is also described to integrate the microcapillaries with a sample-injection cross without the requirement of glass etching. The 10-mm-long microcapillaries sustain field intensities up to 90 kV/m and limit the temperature excursions due to Joule heating to a few degrees Celsius only.",

author = "Zhen Cao and Kangning REN and Hongkai Wu and Levent Yobas",

note = "This project was financially supported in part by the Startup Grant from the ECE Department, HKUST, the Research Project Competition Grant by the HKUST (No. RPC11EG09) and the Research Grant Council of Hong Kong, a Direct Allocation Grant to HKUST (No. DAG09/10.EG09). ",

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AU - Cao, Zhen

AU - REN, Kangning

AU - Wu, Hongkai

AU - Yobas, Levent

N1 - This project was financially supported in part by the Startup Grant from the ECE Department, HKUST, the Research Project Competition Grant by the HKUST (No. RPC11EG09) and the Research Grant Council of Hong Kong, a Direct Allocation Grant to HKUST (No. DAG09/10.EG09).

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N2 - We demonstrate monolithic integration of fine cylindrical glass microcapillaries (diameter ~1 μm) on silicon and evaluate their performance for electrophoretic separation of biomolecules. Such microcapillaries are achieved through thermal reflow of a glass layer on microstructured silicon whereby slender voids are moulded into cylindrical tubes. The process allows self-enclosed microcapillaries with a uniform profile. A simplified method is also described to integrate the microcapillaries with a sample-injection cross without the requirement of glass etching. The 10-mm-long microcapillaries sustain field intensities up to 90 kV/m and limit the temperature excursions due to Joule heating to a few degrees Celsius only.

AB - We demonstrate monolithic integration of fine cylindrical glass microcapillaries (diameter ~1 μm) on silicon and evaluate their performance for electrophoretic separation of biomolecules. Such microcapillaries are achieved through thermal reflow of a glass layer on microstructured silicon whereby slender voids are moulded into cylindrical tubes. The process allows self-enclosed microcapillaries with a uniform profile. A simplified method is also described to integrate the microcapillaries with a sample-injection cross without the requirement of glass etching. The 10-mm-long microcapillaries sustain field intensities up to 90 kV/m and limit the temperature excursions due to Joule heating to a few degrees Celsius only.

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Monolithic integration of fine cylindrical glass microcapillaries on silicon for electrophoretic separation of biomolecules

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