Monitoring of DNA-protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy

Kin Man Lo; Chung Yin Lai; Ho Man Chan; Edmond Dik Lung MA; Hung Wing LI

doi:10.1016/j.ymeth.2013.08.002

Monitoring of DNA-protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy

Kin Man Lo, Chung Yin Lai, Ho Man Chan, Edmond Dik Lung MA, Hung Wing LI^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λ_max shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement.

Original language	English
Pages (from-to)	331-337
Number of pages	7
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.08.002
Publication status	Published - 15 Dec 2013

Scopus Subject Areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

User-Defined Keywords

LSPR
Protein-DNA interactions
Single nanoparticles

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10.1016/j.ymeth.2013.08.002

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title = "Monitoring of DNA-protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy",

abstract = "We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λmax shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement.",

keywords = "LSPR, Protein-DNA interactions, Single nanoparticles",

author = "Lo, {Kin Man} and Lai, {Chung Yin} and Chan, {Ho Man} and MA, {Edmond Dik Lung} and LI, {Hung Wing}",

note = "Funding Information: This work is supported by the Research Grant Councils of the Hong Kong Special Administrative Region ( HKBU201309 ).",

year = "2013",

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doi = "10.1016/j.ymeth.2013.08.002",

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T1 - Monitoring of DNA-protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy

AU - Lo, Kin Man

AU - Lai, Chung Yin

AU - Chan, Ho Man

AU - MA, Edmond Dik Lung

AU - LI, Hung Wing

N1 - Funding Information: This work is supported by the Research Grant Councils of the Hong Kong Special Administrative Region ( HKBU201309 ).

PY - 2013/12/15

Y1 - 2013/12/15

N2 - We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λmax shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement.

AB - We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λmax shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement.

KW - LSPR

KW - Protein-DNA interactions

KW - Single nanoparticles

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DO - 10.1016/j.ymeth.2013.08.002

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SN - 1046-2023

VL - 64

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EP - 337

JO - Methods

JF - Methods

IS - 3

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Monitoring of DNA-protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy

Abstract

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