TY - JOUR
T1 - Molecular mechanisms of survival and apoptosis in RAW 264.7 macrophages under oxidative stress
AU - Zhang, Y.
AU - Fong, C. C.
AU - Wong, M. S.
AU - Tzang, C. H.
AU - Lai, W. P.
AU - Fong, David W F
AU - Sui, S. F.
AU - Yang, M.
N1 - Funding Information:
The work described in this paper was supported by the Areas of Excellent Scheme established under the University Grant Committee of the Hong Kong SAR Government (Project No: AOE/P-04/2004). M. Yang also acknowledges a senior visiting professorship of Tsinghua University provided by Ministry of Education, P.R.C.
PY - 2005/5
Y1 - 2005/5
N2 - Organisms living in an aerobic environment are continuously exposed to reactive oxygen species (ROS). Apoptosis of cells can be induced by ROS and cells also develop negative feedback mechanisms to limit ROS induced cell death. In this study, RAW264.7 murine macrophage cells were treated with H 2O2 and cDNA microarray technique was used to produce gene expression profiles. We found that H2O2 treatment caused up-regulation of stress, survival and apoptosis related genes, and down-regulation of growth and cell cycle promoting genes. Numerous genes of metabolism pathways showed special expression patterns under oxidative stress: glycolysis and lipid synthesis related genes were down-regulated whereas the genes of lipid catabolism and protein synthesis were up-regulated. We also identified several signaling molecules as ROS-responsive, including p53, Akt, NF-κ B, ERK, JNK, p38, PKC and INF-γ . They played important roles in the process of apoptosis or cell survival. Finally, an interactive pathway involved in cellular response to oxidative stress was proposed to provide some insight into the molecular events of apoptosis induced by ROS and the feedback mechanisms involved in cell survival.
AB - Organisms living in an aerobic environment are continuously exposed to reactive oxygen species (ROS). Apoptosis of cells can be induced by ROS and cells also develop negative feedback mechanisms to limit ROS induced cell death. In this study, RAW264.7 murine macrophage cells were treated with H 2O2 and cDNA microarray technique was used to produce gene expression profiles. We found that H2O2 treatment caused up-regulation of stress, survival and apoptosis related genes, and down-regulation of growth and cell cycle promoting genes. Numerous genes of metabolism pathways showed special expression patterns under oxidative stress: glycolysis and lipid synthesis related genes were down-regulated whereas the genes of lipid catabolism and protein synthesis were up-regulated. We also identified several signaling molecules as ROS-responsive, including p53, Akt, NF-κ B, ERK, JNK, p38, PKC and INF-γ . They played important roles in the process of apoptosis or cell survival. Finally, an interactive pathway involved in cellular response to oxidative stress was proposed to provide some insight into the molecular events of apoptosis induced by ROS and the feedback mechanisms involved in cell survival.
KW - Apoptosis and survival
KW - cDNA microarray
KW - Hydrogen peroxide
KW - RAW 264.7 macrophages
KW - Reactive oxygen Species
UR - https://www.scopus.com/pages/publications/21244437180
U2 - 10.1007/s10495-005-1885-0
DO - 10.1007/s10495-005-1885-0
M3 - Journal article
C2 - 15909117
AN - SCOPUS:21244437180
SN - 1360-8185
VL - 10
SP - 545
EP - 556
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
IS - 3
ER -
