Molecular mechanisms of survival and apoptosis in RAW 264.7 macrophages under oxidative stress

Y. Zhang, C. C. Fong, M. S. Wong, C. H. Tzang, W. P. Lai, David W F FONG, S. F. Sui, M. Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Organisms living in an aerobic environment are continuously exposed to reactive oxygen species (ROS). Apoptosis of cells can be induced by ROS and cells also develop negative feedback mechanisms to limit ROS induced cell death. In this study, RAW264.7 murine macrophage cells were treated with H 2O2 and cDNA microarray technique was used to produce gene expression profiles. We found that H2O2 treatment caused up-regulation of stress, survival and apoptosis related genes, and down-regulation of growth and cell cycle promoting genes. Numerous genes of metabolism pathways showed special expression patterns under oxidative stress: glycolysis and lipid synthesis related genes were down-regulated whereas the genes of lipid catabolism and protein synthesis were up-regulated. We also identified several signaling molecules as ROS-responsive, including p53, Akt, NF-κ B, ERK, JNK, p38, PKC and INF-γ . They played important roles in the process of apoptosis or cell survival. Finally, an interactive pathway involved in cellular response to oxidative stress was proposed to provide some insight into the molecular events of apoptosis induced by ROS and the feedback mechanisms involved in cell survival.

Original languageEnglish
Pages (from-to)545-556
Number of pages12
JournalApoptosis : an international journal on programmed cell death
Volume10
Issue number3
DOIs
Publication statusPublished - May 2005

Scopus Subject Areas

  • Pharmacology
  • Pharmaceutical Science
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Cancer Research

User-Defined Keywords

  • Apoptosis and survival
  • cDNA microarray
  • Hydrogen peroxide
  • RAW 264.7 macrophages
  • Reactive oxygen Species

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