Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

Guoyin Kai; Pan Liao; Hui Xu; Jing Wang; Congcong Zhou; Wei Zhou; Yaping Qi; Jianbo Xiao; Yuliang Wang; Lin Zhang

doi:10.1007/s11738-012-0940-z

Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

Guoyin Kai^*, Pan Liao, Hui Xu, Jing Wang, Congcong Zhou, Wei Zhou, Yaping Qi, Jianbo Xiao, Yuliang Wang, Lin Zhang

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

74 Citations (Scopus)

Abstract

To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.

Original language	English
Pages (from-to)	1421–1433
Number of pages	13
Journal	Acta Physiologiae Plantarum
Volume	34
Issue number	4
DOIs	https://doi.org/10.1007/s11738-012-0940-z
Publication status	Published - 1 Jul 2012

Scopus Subject Areas

Physiology
Agronomy and Crop Science
Plant Science

User-Defined Keywords

Bioactive compounds
Dan Shen
Elicitors
Gene expression
Metabolic engineering

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10.1007/s11738-012-0940-z

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@article{618b1e3ef3ab451fb8132138e195c2db,

title = "Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures",

abstract = "To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.",

keywords = "Bioactive compounds, Dan Shen, Elicitors, Gene expression, Metabolic engineering",

author = "Guoyin Kai and Pan Liao and Hui Xu and Jing Wang and Congcong Zhou and Wei Zhou and Yaping Qi and Jianbo Xiao and Yuliang Wang and Lin Zhang",

note = "Funding Information: This work was supported by National Natural Science Fund (30900110), Shanghai Science and Technology Committee Project (10JC1412000, 09QH1401900, 06QA14038, 08391911800, 073158202, 075405117, 065458022, 05ZR14093), Project from Ministry of Science and Technology of China (NC2010AE0075, NC2010AE0372), Zhejiang Provincial Natural Science Fund (Y2080621), Shanghai Education Committee Fund (09ZZ138, 06DZ015, J50401), Fujian Science and Technology Committee Key Special Project (2008NZ0001-4), National Transgenic Organism New Variety Culture Key Project (2009ZX08012-002B), Project from Shanghai Normal University (SK201230, SK201236, SK200830). We gratefully thank Kyle Andrew Schneider (University of Dayton, Ohio, USA) for grammatical correction of the manuscript. Publisher Copyright: {\textcopyright} Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w 2012.",

year = "2012",

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doi = "10.1007/s11738-012-0940-z",

language = "English",

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T1 - Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

AU - Kai, Guoyin

AU - Liao, Pan

AU - Xu, Hui

AU - Wang, Jing

AU - Zhou, Congcong

AU - Zhou, Wei

AU - Qi, Yaping

AU - Xiao, Jianbo

AU - Wang, Yuliang

AU - Zhang, Lin

N1 - Funding Information: This work was supported by National Natural Science Fund (30900110), Shanghai Science and Technology Committee Project (10JC1412000, 09QH1401900, 06QA14038, 08391911800, 073158202, 075405117, 065458022, 05ZR14093), Project from Ministry of Science and Technology of China (NC2010AE0075, NC2010AE0372), Zhejiang Provincial Natural Science Fund (Y2080621), Shanghai Education Committee Fund (09ZZ138, 06DZ015, J50401), Fujian Science and Technology Committee Key Special Project (2008NZ0001-4), National Transgenic Organism New Variety Culture Key Project (2009ZX08012-002B), Project from Shanghai Normal University (SK201230, SK201236, SK200830). We gratefully thank Kyle Andrew Schneider (University of Dayton, Ohio, USA) for grammatical correction of the manuscript. Publisher Copyright: © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012.

PY - 2012/7/1

Y1 - 2012/7/1

N2 - To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.

AB - To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.

KW - Bioactive compounds

KW - Dan Shen

KW - Elicitors

KW - Gene expression

KW - Metabolic engineering

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DO - 10.1007/s11738-012-0940-z

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AN - SCOPUS:84856386039

SN - 0137-5881

VL - 34

SP - 1421

EP - 1433

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

IS - 4

ER -

Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

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