MNSs-mediated N-glycan processing is essential for auxin homeostasis in Arabidopsis roots during alkaline response

Tianyu Xia, Yujie Zhan, Yangjie Mu, Jianhua Zhang, Weifeng Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Early steps in the endoplasmic reticulum (ER) lumen and cis-Golgi comprise trimming of N-glycans by class I α-mannosidases (MNSs) play crucial roles in root growth and stress response. Herein, we found that the root growth inhibition in the mns1 mns2 mns3 mutant was partially rescued under alkaline condition, and inhibitor treatment to disrupt auxin transport counteracted this alkaline-maintained root growth. Further study showed that indole-3-acetic acid (IAA) levels were undetectable in mns1 mns2 mns3 at normal condition and recovered at alkaline condition, which corroborate our N-glycopeptide profiling, from which N-glycopeptides related with IAA biosynthesis, amino acid conjugates hydrolysis, and response showed differential abundance between normal and alkaline conditions in mns1 mns2 mns3. Overall, our results linked the need for MNSs-mediated N-glycan processing in the ER and cis-Golgi with maintenance of auxin homeostasis and transport in Arabidopsis roots during the response to alkaline stress.

Original languageEnglish
Article number104298
Number of pages16
JournaliScience
Volume25
Issue number5
DOIs
Publication statusPublished - 20 May 2022

Scopus Subject Areas

  • General

User-Defined Keywords

  • Biological sciences
  • Plant biology
  • Plant physiology
  • Plants

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