TY - JOUR
T1 - Microfluidic-based metal enhanced fluorescence for capillary electrophoresis by Ag nanorod arrays
AU - Xiao, Chenyu
AU - Cao, Zhen
AU - Deng, Junhong
AU - HUANG, Jeffery
AU - Xu, Zheng
AU - FU, Junxue
AU - Yobas, Levent
N1 - Publisher Copyright:
© 2014 IOP Publishing Ltd.
PY - 2014/5/15
Y1 - 2014/5/15
N2 - As metal nanorods show much higher metal enhanced fluorescence (MEF) than metal nanospheres, microfluidic-based MEF is first explored with Ag nanorod (ND) arrays made by oblique angle deposition. By measuring the fluorescein isothiocyanate (FITC) solution sandwiched between the Ag NDs and a piece of cover slip, the enhancement factors (EFs) are found as 3.7 ± 0.64 and 6.74 ± 2.04, for a solution thickness at 20.8 μm and 10 μm, respectively. Because of the strong plasmonic coupling between the adjacent Ag NDs, only the emission of the fluorophores present in the three-dimensional NDs array gets enhanced. Thus, the corresponding effective enhancement factors (EEFs) are revealed to be relatively close, 259 ± 92 and 340 ± 102, respectively. To demonstrate the application of MEF in microfluidic systems, a multilayer of SiO2 NDs/Ag NDs is integrated with a capillary electrophoresis device. At a microchannel depth of 10 μm, an enhancement of 6.5 fold is obtained for amino acids separation detection. These results are very encouraging and open the possibility of MEF applications for the Ag ND arrays decorated microchannels. With the miniaturization of microfluidic devices, microfluidic-based MEF by Ag ND arrays will likely find more applications with further enhancement.
AB - As metal nanorods show much higher metal enhanced fluorescence (MEF) than metal nanospheres, microfluidic-based MEF is first explored with Ag nanorod (ND) arrays made by oblique angle deposition. By measuring the fluorescein isothiocyanate (FITC) solution sandwiched between the Ag NDs and a piece of cover slip, the enhancement factors (EFs) are found as 3.7 ± 0.64 and 6.74 ± 2.04, for a solution thickness at 20.8 μm and 10 μm, respectively. Because of the strong plasmonic coupling between the adjacent Ag NDs, only the emission of the fluorophores present in the three-dimensional NDs array gets enhanced. Thus, the corresponding effective enhancement factors (EEFs) are revealed to be relatively close, 259 ± 92 and 340 ± 102, respectively. To demonstrate the application of MEF in microfluidic systems, a multilayer of SiO2 NDs/Ag NDs is integrated with a capillary electrophoresis device. At a microchannel depth of 10 μm, an enhancement of 6.5 fold is obtained for amino acids separation detection. These results are very encouraging and open the possibility of MEF applications for the Ag ND arrays decorated microchannels. With the miniaturization of microfluidic devices, microfluidic-based MEF by Ag ND arrays will likely find more applications with further enhancement.
KW - Ag nanorod
KW - Capillary electrophoresis
KW - Metal enhanced fluorescence
UR - http://www.scopus.com/inward/record.url?scp=84918572960&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/25/22/225502
DO - 10.1088/0957-4484/25/22/225502
M3 - Journal article
C2 - 24833562
AN - SCOPUS:84918572960
SN - 0957-4484
VL - 25
JO - Nanotechnology
JF - Nanotechnology
IS - 22
M1 - 225502
ER -