TY - JOUR
T1 - Microcalorimetric investigation of the effect of non-ionic surfactant on biodegradation of pyrene by PAH-degrading bacteria Burkholderia cepacia
AU - Chen, Ke
AU - Zhu, Qing
AU - Qian, Yiguang
AU - Song, Ying
AU - Yao, Jun
AU - Choi, Martin M.F.
N1 - Funding Information:
This work is supported in part by grants from the International Joint Key Project from Chinese Ministry of Science and Technology ( 2010DFB23160 ), International Joint Key Project from National Natural Science Foundation of China ( 40920134003 ), National Natural Science Foundation of China ( 41273092 ) and National Outstanding Youth Research Foundation of China ( 40925010 ).
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenecity and carcinogenicity. Surfactant has become a hot topic for its wide application in the bioremediation of PAHs. The aim of this work is to explore a microcalorimetric method to determine the toxic effect of pyrene on Bacillus subtilis (B. subtilis) and the PAH-degrading bacteria Burkholderia cepacia (B. cepacia) and to evaluate the effect of Tween 80 on biodegradation of pyrene. Power-time curves were studied and calorimetric parameters including the growth rate constant (k), half inhibitory concentration (IC50), and total thermal effect (QT) were determined. B. subtilis, B. cepacia and B. cepacia with Tween 80 were completely inhibited when the concentration of pyrene were 200, 800 and 1600μgmL-1, respectively. B. cepacia shows better tolerance to pyrene than B. subtilis. Tween 80 significantly improves the biodegradation of pyrene by increasing the bioavailability of pyrene. In addition, the expression of catechol 2,3-dioxygenase (C23O) in B. cepacia is responsible for the degradation of pyrene and plays an important role in improving the biodegradation of pyrene. Moreover, the activity of C23O increases with the application of Tween 80. The enhanced bioavailability and biodegradation of pyrene by Tween 80 shows the potential use of Tween 80 in the PAHs bioremediation.
AB - Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenecity and carcinogenicity. Surfactant has become a hot topic for its wide application in the bioremediation of PAHs. The aim of this work is to explore a microcalorimetric method to determine the toxic effect of pyrene on Bacillus subtilis (B. subtilis) and the PAH-degrading bacteria Burkholderia cepacia (B. cepacia) and to evaluate the effect of Tween 80 on biodegradation of pyrene. Power-time curves were studied and calorimetric parameters including the growth rate constant (k), half inhibitory concentration (IC50), and total thermal effect (QT) were determined. B. subtilis, B. cepacia and B. cepacia with Tween 80 were completely inhibited when the concentration of pyrene were 200, 800 and 1600μgmL-1, respectively. B. cepacia shows better tolerance to pyrene than B. subtilis. Tween 80 significantly improves the biodegradation of pyrene by increasing the bioavailability of pyrene. In addition, the expression of catechol 2,3-dioxygenase (C23O) in B. cepacia is responsible for the degradation of pyrene and plays an important role in improving the biodegradation of pyrene. Moreover, the activity of C23O increases with the application of Tween 80. The enhanced bioavailability and biodegradation of pyrene by Tween 80 shows the potential use of Tween 80 in the PAHs bioremediation.
KW - Biodegradation
KW - Burkholderia cepacia
KW - Microcalorimetry
KW - Non-ionic surfactant
KW - Pyrene
UR - http://www.scopus.com/inward/record.url?scp=84887154838&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2013.08.012
DO - 10.1016/j.ecoenv.2013.08.012
M3 - Journal article
C2 - 24011930
AN - SCOPUS:84887154838
SN - 0147-6513
VL - 98
SP - 361
EP - 367
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -
