Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups

An Xin Hou*, Zhi Xue, Yi Liu, Song Sheng Qu, Rick W K WONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The antibacterial activities towards Escherichia coli of two cationic YbIII-monoporphyrin complexes, [YbIII(TMP)(H2O)3]Cl (1) and [YbIII(TTP)(H2OP)3]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC50 values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herringsperm DNA (HS-DNA), though by different binding modes.

Original languageEnglish
Pages (from-to)2889-2899
Number of pages11
JournalChemistry and Biodiversity
Volume4
Issue number12
DOIs
Publication statusPublished - 2007

Scopus Subject Areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Molecular Medicine
  • Molecular Biology

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