Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups

An Xin Hou; Zhi Xue; Yi Liu; Song Sheng Qu; Rick W K WONG

doi:10.1002/cbdv.200790238

Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups

An Xin Hou^*, Zhi Xue, Yi Liu, Song Sheng Qu, Rick W K WONG

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

The antibacterial activities towards Escherichia coli of two cationic Yb^III-monoporphyrin complexes, [Yb^III(TMP)(H₂O)₃]Cl (1) and [Yb^III(TTP)(H₂OP)₃]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC₅₀ values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herringsperm DNA (HS-DNA), though by different binding modes.

Original language	English
Pages (from-to)	2889-2899
Number of pages	11
Journal	Chemistry and Biodiversity
Volume	4
Issue number	12
DOIs	https://doi.org/10.1002/cbdv.200790238
Publication status	Published - 2007

Scopus Subject Areas

Bioengineering
Biochemistry
Chemistry(all)
Molecular Medicine
Molecular Biology

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title = "Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups",

abstract = "The antibacterial activities towards Escherichia coli of two cationic YbIII-monoporphyrin complexes, [YbIII(TMP)(H2O)3]Cl (1) and [YbIII(TTP)(H2OP)3]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC50 values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herringsperm DNA (HS-DNA), though by different binding modes.",

author = "Hou, {An Xin} and Zhi Xue and Yi Liu and Qu, {Song Sheng} and WONG, {Rick W K}",

year = "2007",

doi = "10.1002/cbdv.200790238",

language = "English",

volume = "4",

pages = "2889--2899",

journal = "Chemistry and Biodiversity",

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T1 - Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups

AU - Hou, An Xin

AU - Xue, Zhi

AU - Liu, Yi

AU - Qu, Song Sheng

AU - WONG, Rick W K

PY - 2007

Y1 - 2007

N2 - The antibacterial activities towards Escherichia coli of two cationic YbIII-monoporphyrin complexes, [YbIII(TMP)(H2O)3]Cl (1) and [YbIII(TTP)(H2OP)3]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC50 values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herringsperm DNA (HS-DNA), though by different binding modes.

AB - The antibacterial activities towards Escherichia coli of two cationic YbIII-monoporphyrin complexes, [YbIII(TMP)(H2O)3]Cl (1) and [YbIII(TTP)(H2OP)3]Cl (2), were investigated at the cellular and sub-cellular levels. The biological effects of the complexes on the growth of E. coli were evaluated by microcalorimetry and by analysis of the resulting metabolic thermogenic curves, from which IC50 values and metabolic parameters such as growth rate and generation time were derived. At the subcellular level, DNA-binding experiments were performed by means of UV/VIS- and fluorescence-titration experiments, as well as by near-infrared (NIR) emission, which revealed that 1 and 2 strongly bind to herringsperm DNA (HS-DNA), though by different binding modes.

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U2 - 10.1002/cbdv.200790238

DO - 10.1002/cbdv.200790238

M3 - Journal article

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AN - SCOPUS:38049067162

SN - 1612-1872

VL - 4

SP - 2889

EP - 2899

JO - Chemistry and Biodiversity

JF - Chemistry and Biodiversity

IS - 12

ER -

Microcalorimetric and spectroscopic investigation of the antibacterial properties of cationic ytterbium(III)-porphyrin complexes lacking charged peripheral groups

Abstract

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