TY - JOUR
T1 - Microbial communities and bacterial diversity of spruce, hemlock and grassland soils of tatachia forest, Taiwan
AU - Selvam, Ammaiyappan
AU - Tsai, Shu Hsien
AU - Liu, Ching Piao
AU - Chen, I. Chu
AU - Chang, Cheng Hsiung
AU - Yang, Shang Shyng
N1 - Funding Information:
The authors thank the National Science Council of Taiwan for financial supports (NSC 94-2313-B002-090, NSC 95-2313-B002-040, and NSC 96-2313-B002-020).
PY - 2010/7
Y1 - 2010/7
N2 - To evaluate the bacterial diversity of Tatachia Forest soils, 16S rDNA clone libraries of the spruce, hemlock and grassland soils were constructed. Further, the influence of physicochemical and biological properties of soil on microbial ecology, pH, moisture content, microbial population and biomass were also analyzed. The soil pH increased with the increasing of soil depth; whereas the microbial population, biomass, moisture content, total organic carbon and total nitrogen were reverse. Microbial populations were the highest in the summer season which also correlated with the highest moisture content. The phylogenetic analyses revealed that the clones from nine 16S rDNA clone libraries represented Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Verrucomicrobia, candidate division TG1 and candidate division TM7.Members of Proteobacteria, Acidobacteria and Actinobacteria constituted 42.2, 35.1 and 7.8% of the clone libraries, respectively; whereas the remaining bacterial divisions each comprised <3%. The spruce site had the highest bacterial diversity among the tested sites, followed by the hemlock sites and the grassland sites with the least. The bacterial community is the more diverse in the organic layer than that in deeper horizons. Further, bacterial diversity through the gradient horizons was different, indicating that the bacterial diversity in the deeper horizons is not simply the diluted analogs of the surface soils and some microbes dominate only in the deeper horizons.
AB - To evaluate the bacterial diversity of Tatachia Forest soils, 16S rDNA clone libraries of the spruce, hemlock and grassland soils were constructed. Further, the influence of physicochemical and biological properties of soil on microbial ecology, pH, moisture content, microbial population and biomass were also analyzed. The soil pH increased with the increasing of soil depth; whereas the microbial population, biomass, moisture content, total organic carbon and total nitrogen were reverse. Microbial populations were the highest in the summer season which also correlated with the highest moisture content. The phylogenetic analyses revealed that the clones from nine 16S rDNA clone libraries represented Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Verrucomicrobia, candidate division TG1 and candidate division TM7.Members of Proteobacteria, Acidobacteria and Actinobacteria constituted 42.2, 35.1 and 7.8% of the clone libraries, respectively; whereas the remaining bacterial divisions each comprised <3%. The spruce site had the highest bacterial diversity among the tested sites, followed by the hemlock sites and the grassland sites with the least. The bacterial community is the more diverse in the organic layer than that in deeper horizons. Further, bacterial diversity through the gradient horizons was different, indicating that the bacterial diversity in the deeper horizons is not simply the diluted analogs of the surface soils and some microbes dominate only in the deeper horizons.
KW - 16s rdna clone library
KW - Bacterial diversity
KW - Microbial biomass carbon and nitrogen
KW - Microbial population
UR - http://www.scopus.com/inward/record.url?scp=77953160105&partnerID=8YFLogxK
U2 - 10.1080/03601231003799960
DO - 10.1080/03601231003799960
M3 - Journal article
C2 - 20512729
AN - SCOPUS:77953160105
SN - 0360-1234
VL - 45
SP - 386
EP - 398
JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
IS - 5
ER -