Microbial communities and bacterial diversity of spruce, hemlock and grassland soils of tatachia forest, Taiwan

Ammaiyappan Selvam, Shu Hsien Tsai, Ching Piao Liu, I. Chu Chen, Cheng Hsiung Chang, Shang Shyng Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

To evaluate the bacterial diversity of Tatachia Forest soils, 16S rDNA clone libraries of the spruce, hemlock and grassland soils were constructed. Further, the influence of physicochemical and biological properties of soil on microbial ecology, pH, moisture content, microbial population and biomass were also analyzed. The soil pH increased with the increasing of soil depth; whereas the microbial population, biomass, moisture content, total organic carbon and total nitrogen were reverse. Microbial populations were the highest in the summer season which also correlated with the highest moisture content. The phylogenetic analyses revealed that the clones from nine 16S rDNA clone libraries represented Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Verrucomicrobia, candidate division TG1 and candidate division TM7.Members of Proteobacteria, Acidobacteria and Actinobacteria constituted 42.2, 35.1 and 7.8% of the clone libraries, respectively; whereas the remaining bacterial divisions each comprised <3%. The spruce site had the highest bacterial diversity among the tested sites, followed by the hemlock sites and the grassland sites with the least. The bacterial community is the more diverse in the organic layer than that in deeper horizons. Further, bacterial diversity through the gradient horizons was different, indicating that the bacterial diversity in the deeper horizons is not simply the diluted analogs of the surface soils and some microbes dominate only in the deeper horizons.

Original languageEnglish
Pages (from-to)386-398
Number of pages13
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume45
Issue number5
DOIs
Publication statusPublished - Jul 2010

Scopus Subject Areas

  • Food Science
  • Pollution

User-Defined Keywords

  • 16s rdna clone library
  • Bacterial diversity
  • Microbial biomass carbon and nitrogen
  • Microbial population

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