Microarrays for the Study of Viral Gene Expression During Human Cytomegalovirus Latent Infection

Barry Slobedman, Allen K. L. Cheung

Research output: Chapter in book/report/conference proceedingChapterpeer-review

Abstract

Human cytomegalovirus (HCMV) is one of the largest known DNA viruses. It is ubiquitous, and following resolution of primary productive infection, it persists in the human host by establishing a lifelong latent infection in myeloid lineage cells such as monocytes and their progenitors. Most adults with HCMV infection are healthy but it can cause neurologic deficits in infants, and remains an important cause of morbidity and mortality in the immunosuppressed patient. Microarray-based studies of HCMV have provided useful information about genes that are transcriptionally active during both productive and latent phases of infection. This chapter describes how to study genes in HCMV using microarrays and two cell types (productively infected human foreskin fibroblasts, and latently infected primary human myeloid progenitor cells).
Original languageEnglish
Title of host publicationClinical Bioinformatics
EditorsRonald J. A. Trent
PublisherHumana Totowa, NJ
Pages153–175
Number of pages23
Edition1st
ISBN (Electronic)9781603271486
ISBN (Print)9781588297914, 9781617377815
DOIs
Publication statusPublished - 2008

Publication series

NameMethods in Molecular Medicine
ISSN (Print)1543-1894
ISSN (Electronic)1940-6037

User-Defined Keywords

  • microarray
  • herpesvirus human cytomegalovirus latent infection
  • myeloid progenitor cell
  • viral gene transcription

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