Micelle-mediated extraction and preconcentration of ginsenosides from Chinese herbal medicine

Qun Fang, Hin Wing Yeung, Hei Wun Leung, Carmen W K HUIE*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

74 Citations (Scopus)


The feasibility of employing micelle-mediated extraction as an alternative and effective method for the solubilization, purification and/or preconcentration of active ingredients from herbal products is demonstrated for the first time using the root of American ginseng as a model. When compared to methanol and water, an aqueous surfactant solution containing 10% Triton X-100 yielded faster kinetics and higher recovery for the extraction of various ginsenosides. An experimental design approach (uniform design) was demonstrated as a novel and useful method for the optimization of experimental factors involved in the micelle-mediated extraction process. For the preconcentration of ginsenosides prior to chromatographic determination, a salting-out agent (sodium sulfate) was employed to make the efficient cloud point extraction of both hydrophobic and hydrophilic ginsenosides into the surfactant-rich phase possible, as well as to increase the preconcentration factor by reducing the volume of the surfactant-rich phase. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalJournal of Chromatography A
Issue number1
Publication statusPublished - 22 Dec 2000

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Cloud point extraction
  • Ginsenosides
  • Micelle-mediated extraction


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