Methods for analysis of disease resistance and the defense response in arabidopsis

Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, Yiji XIA

Research output: Chapter in book/report/conference proceedingChapterpeer-review

3 Citations (Scopus)


Many G proteins are involved in the plant defense responses against pathogen infection. With Arabidopsis as a model, this chapter describes the protocols commonly used for inoculating plants with Pseudomonas syringae (a bacterial pathogen) and Botrytis cinerea (a fungal pathogen) for analyzing disease resistance phenotypes caused by these pathogens. In addition, the procedures are provided for observation of the hypersensitive response triggered by avirulent strains of P. syringae and for analyzing pathogen-responsive gene expression and MAPK activation.

Original languageEnglish
Title of host publicationG Protein-Coupled Receptor Signaling in Plants
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages12
ISBN (Print)9781627035316
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Scopus Subject Areas

  • Molecular Biology
  • Genetics

User-Defined Keywords

  • Botrytis cinerea
  • MAPK activity assay
  • Plant disease resistance
  • Pseudomonas syringae
  • The hypersensitive response


Dive into the research topics of 'Methods for analysis of disease resistance and the defense response in arabidopsis'. Together they form a unique fingerprint.

Cite this