Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study

Kang Yun Lee; Chris Kong Chu Wong; Kai Jen Chuang; Mauo Ying Bien; Jun Ji Cao; Yong Ming Han; Linwei Tian; Chih Cheng Chang; Po Hao Feng; Kin Fai Ho; Hsiao Chi Chuang

doi:10.1016/j.jhazmat.2014.04.029

Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study

Kang Yun Lee, Chris Kong Chu Wong, Kai Jen Chuang, Mauo Ying Bien, Jun Ji Cao, Yong Ming Han, Linwei Tian, Chih Cheng Chang, Po Hao Feng, Kin Fai Ho^*, Hsiao Chi Chuang

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

32 Citations (Scopus)

Abstract

The potential effects of inhaled fine particulate matter (PM_2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM_2.5 from haze episodes on protein oxidation. PM_2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM_2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM_2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM_2.5 increased oxidative stress. Addition of PM_2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM_2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.

Original language	English
Pages (from-to)	384-391
Number of pages	8
Journal	Journal of Hazardous Materials
Volume	274
DOIs	https://doi.org/10.1016/j.jhazmat.2014.04.029
Publication status	Published - 15 Jun 2014

Scopus Subject Areas

Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
Health, Toxicology and Mutagenesis

User-Defined Keywords

Air pollution
Liquid chromatography-mass spectrometry
Polycyclic aromatic hydrocarbons
Protein structure
Pulmonary

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10.1016/j.jhazmat.2014.04.029

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@article{d85e630c2b29408f9d227c087e22fd6f,

title = "Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study",

abstract = "The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.",

keywords = "Air pollution, Liquid chromatography-mass spectrometry, Polycyclic aromatic hydrocarbons, Protein structure, Pulmonary",

author = "Lee, {Kang Yun} and Wong, {Chris Kong Chu} and Chuang, {Kai Jen} and Bien, {Mauo Ying} and Cao, {Jun Ji} and Han, {Yong Ming} and Linwei Tian and Chang, {Chih Cheng} and Feng, {Po Hao} and Ho, {Kin Fai} and Chuang, {Hsiao Chi}",

note = "Funding Information: This study was founded by the Taipei Medical University and Shuang Ho Hospital (TMU101-AE1-B53 and 102TMU-SHH-09), Research Grants Council of the Hong Kong Special Administrative Region China (Project No. CUHK 412612), the National Natural Science Foundation of China (41230641) and the Strategic Priority Research Program of the Chinese Academy of Science (XDA05100401). ",

year = "2014",

month = jun,

day = "15",

doi = "10.1016/j.jhazmat.2014.04.029",

language = "English",

volume = "274",

pages = "384--391",

journal = "Journal of Hazardous Materials",

issn = "0304-3894",

publisher = "Elsevier",

}

TY - JOUR

T1 - Methionine oxidation in albumin by fine haze particulate matter

T2 - An in vitro and in vivo study

AU - Lee, Kang Yun

AU - Wong, Chris Kong Chu

AU - Chuang, Kai Jen

AU - Bien, Mauo Ying

AU - Cao, Jun Ji

AU - Han, Yong Ming

AU - Tian, Linwei

AU - Chang, Chih Cheng

AU - Feng, Po Hao

AU - Ho, Kin Fai

AU - Chuang, Hsiao Chi

N1 - Funding Information: This study was founded by the Taipei Medical University and Shuang Ho Hospital (TMU101-AE1-B53 and 102TMU-SHH-09), Research Grants Council of the Hong Kong Special Administrative Region China (Project No. CUHK 412612), the National Natural Science Foundation of China (41230641) and the Strategic Priority Research Program of the Chinese Academy of Science (XDA05100401).

PY - 2014/6/15

Y1 - 2014/6/15

N2 - The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.

AB - The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.

KW - Air pollution

KW - Liquid chromatography-mass spectrometry

KW - Polycyclic aromatic hydrocarbons

KW - Protein structure

KW - Pulmonary

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U2 - 10.1016/j.jhazmat.2014.04.029

DO - 10.1016/j.jhazmat.2014.04.029

M3 - Journal article

C2 - 24801896

AN - SCOPUS:84899723651

SN - 0304-3894

VL - 274

SP - 384

EP - 391

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

ER -

Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study

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