TY - JOUR
T1 - Metal enhanced fluorescence improved protein and DNA detection by zigzag Ag nanorod arrays
AU - Ji, Xiaofan
AU - Xiao, Chenyu
AU - Lau, Wai Fung
AU - Li, Jianping
AU - Fu, Junxue
N1 - Funding Information:
The authors thank the financial support from the Hong Kong UGC General Research Fund HKBU200813, SZSTI JCYJ20130401171812113 and National Natural Science Foundation of China 21303152.
Publisher copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/8/15
Y1 - 2016/8/15
N2 - As metal nano-arrays show great potential on metal enhanced fluorescence (MEF) than random nanostructures, MEF of Ag zigzag nanorod (ZNR) arrays made by oblique angle deposition has been studied for biomolecule-protein interaction and DNA hybridization. By changing the folding number and the deposition substrate temperature, a 14-fold enhancement factor (EF) is obtained for biotin-neutravidin detection. The optimal folding number is decided as Z=7, owing to the high scattering intensity of Ag ZNRs. The substrate temperature T=25 °C and 0 °C slightly alters the morphology of Ag ZNRs but has no big difference in EF. Further, Ag ZNRs deposited on a layer of Ag film have been introduced to the DNA hybridization and a significant signal enhancement has been observed through the fluorescence microscope. Through a detailed quantitative EF analysis, which excludes the enhancing effect from the increased surface area of ZNRs and only considers the contribution of MEF, an EF of 28 is achieved for the hybridization of two single-stranded oligonucleotides with 33 bases. Furthermore, a limit of detection is determined as 0.01 pM. We believe that the Ag ZNR arrays can serve as a universal and sensitive bio-detection platform.
AB - As metal nano-arrays show great potential on metal enhanced fluorescence (MEF) than random nanostructures, MEF of Ag zigzag nanorod (ZNR) arrays made by oblique angle deposition has been studied for biomolecule-protein interaction and DNA hybridization. By changing the folding number and the deposition substrate temperature, a 14-fold enhancement factor (EF) is obtained for biotin-neutravidin detection. The optimal folding number is decided as Z=7, owing to the high scattering intensity of Ag ZNRs. The substrate temperature T=25 °C and 0 °C slightly alters the morphology of Ag ZNRs but has no big difference in EF. Further, Ag ZNRs deposited on a layer of Ag film have been introduced to the DNA hybridization and a significant signal enhancement has been observed through the fluorescence microscope. Through a detailed quantitative EF analysis, which excludes the enhancing effect from the increased surface area of ZNRs and only considers the contribution of MEF, an EF of 28 is achieved for the hybridization of two single-stranded oligonucleotides with 33 bases. Furthermore, a limit of detection is determined as 0.01 pM. We believe that the Ag ZNR arrays can serve as a universal and sensitive bio-detection platform.
KW - Ag zigzag nanorod arrays
KW - Biomolecule-protein interaction
KW - DNA hybridization
KW - Metal enhanced fluorescence
KW - Oblique angle deposition
UR - http://www.scopus.com/inward/record.url?scp=84962859166&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.04.022
DO - 10.1016/j.bios.2016.04.022
M3 - Journal article
C2 - 27088369
AN - SCOPUS:84962859166
SN - 0956-5663
VL - 82
SP - 240
EP - 247
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -