TY - JOUR
T1 - Metagenomic insights into the inhibitory mechanisms of Cu on fermentative hydrogen production
AU - Cao, Jinman
AU - Duan, Guilan
AU - Lin, Aijun
AU - Zhou, Yaoyu
AU - You, Siming
AU - Wong, Jonathan W.C.
AU - Yang, Guang
N1 - Funding Information:
This study was supported by the National Natural Science Foundation of China (No. 41991332), the Key Research and Development Program of Shandong Province, China (No. 2021CXGC010803), and the China Postdoctoral Science Foundation (No. 2022M713307).
Publisher Copyright:
© 2023 Elsevier Ltd. All rights reserved.
PY - 2023/7
Y1 - 2023/7
N2 - Cu is widely present in the feedstocks of dark fermentation, which can inhibit H2 production efficiency of the process. However, current understanding on the inhibitory mechanisms of Cu, especially the microbiological mechanism, is still lacking. This study investigated the inhibitory mechanisms of Cu2+ on fermentative hydrogen production by metagenomics sequencing. Results showed that the exposure to Cu2+ reduced the abundances of high-yielding hydrogen-producing genera (e.g. Clostridium sensu stricto), and remarkably down-regulated the genes involved in substrate membrane transport (e.g., gtsA, gtsB and gtsC), glycolysis (e.g. PK, ppgK and pgi-pmi), and hydrogen formation (e.g. pflA, fdoG, por and E1.12.7.2), leading to significant inhibition on the process performances. The H2 yield was reduced from 1.49 mol H2/mol-glucose to 0.59 and 0.05 mol H2/mol-glucose upon exposure to 500 and 1000 mg/L of Cu2+, respectively. High concentrations of Cu2+ also reduced the rate of H2 production and prolonged the H2-producing lag phase.
AB - Cu is widely present in the feedstocks of dark fermentation, which can inhibit H2 production efficiency of the process. However, current understanding on the inhibitory mechanisms of Cu, especially the microbiological mechanism, is still lacking. This study investigated the inhibitory mechanisms of Cu2+ on fermentative hydrogen production by metagenomics sequencing. Results showed that the exposure to Cu2+ reduced the abundances of high-yielding hydrogen-producing genera (e.g. Clostridium sensu stricto), and remarkably down-regulated the genes involved in substrate membrane transport (e.g., gtsA, gtsB and gtsC), glycolysis (e.g. PK, ppgK and pgi-pmi), and hydrogen formation (e.g. pflA, fdoG, por and E1.12.7.2), leading to significant inhibition on the process performances. The H2 yield was reduced from 1.49 mol H2/mol-glucose to 0.59 and 0.05 mol H2/mol-glucose upon exposure to 500 and 1000 mg/L of Cu2+, respectively. High concentrations of Cu2+ also reduced the rate of H2 production and prolonged the H2-producing lag phase.
KW - Cu
KW - Fermentative hydrogen production
KW - Inhibition
KW - Metagenomic analysis
KW - Microbial community structure
UR - http://www.scopus.com/inward/record.url?scp=85153613543&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.129080
DO - 10.1016/j.biortech.2023.129080
M3 - Journal article
C2 - 37094620
AN - SCOPUS:85153613543
SN - 0960-8524
VL - 380
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 129080
ER -
