Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes

Francesc Sureda; Agata Copani; Valeria Bruno; Thomas Knöpfel; Gainer Meltzger; Ferdinando Nicoletti

doi:10.1016/S0014-2999(97)81950-4

Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes

Francesc Sureda, Agata Copani, Valeria Bruno, Thomas Knöpfel, Gainer Meltzger, Ferdinando Nicoletti^*

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

23 Citations (Scopus)

Abstract

The metabotropic glutamate (mGlu) receptor agonists, quisqualate and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), but not (RS)-3,5-dihydroxyphenylglycine or (2S,3S,4S)-α-(carboxycyclopropyl)glycine stimulated [³H]inositolmonophosphate ([3H]InsP) formation in primary cultures of rat hepatocytes. 1S,3R-ACPD-stimulated [³H]InsP formation was inhibited by α-methyl-4-carboxyphenylglycine, indicating that cultured hepatocytes express functional mGlu receptors coupled to polyphosphoinositide hydrolysis. The identity of these receptors is not similar to that of any of the known mGlu receptor subtypes characterized in heterologous expression systems.

Original language	English
Pages (from-to)	R1-R2
Number of pages	2
Journal	European Journal of Pharmacology
Volume	338
Issue number	2
DOIs	https://doi.org/10.1016/S0014-2999(97)81950-4
Publication status	Published - 5 Nov 1997

User-Defined Keywords

Hepatocyte cultured
Metabotropic glutamate receptor
Polyphosphoinositide hydrolysis

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10.1016/S0014-2999(97)81950-4

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title = "Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes",

abstract = "The metabotropic glutamate (mGlu) receptor agonists, quisqualate and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), but not (RS)-3,5-dihydroxyphenylglycine or (2S,3S,4S)-α-(carboxycyclopropyl)glycine stimulated [3H]inositolmonophosphate ([3H]InsP) formation in primary cultures of rat hepatocytes. 1S,3R-ACPD-stimulated [3H]InsP formation was inhibited by α-methyl-4-carboxyphenylglycine, indicating that cultured hepatocytes express functional mGlu receptors coupled to polyphosphoinositide hydrolysis. The identity of these receptors is not similar to that of any of the known mGlu receptor subtypes characterized in heterologous expression systems.",

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T1 - Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes

AU - Sureda, Francesc

AU - Copani, Agata

AU - Bruno, Valeria

AU - Knöpfel, Thomas

AU - Meltzger, Gainer

AU - Nicoletti, Ferdinando

PY - 1997/11/5

Y1 - 1997/11/5

N2 - The metabotropic glutamate (mGlu) receptor agonists, quisqualate and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), but not (RS)-3,5-dihydroxyphenylglycine or (2S,3S,4S)-α-(carboxycyclopropyl)glycine stimulated [3H]inositolmonophosphate ([3H]InsP) formation in primary cultures of rat hepatocytes. 1S,3R-ACPD-stimulated [3H]InsP formation was inhibited by α-methyl-4-carboxyphenylglycine, indicating that cultured hepatocytes express functional mGlu receptors coupled to polyphosphoinositide hydrolysis. The identity of these receptors is not similar to that of any of the known mGlu receptor subtypes characterized in heterologous expression systems.

AB - The metabotropic glutamate (mGlu) receptor agonists, quisqualate and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), but not (RS)-3,5-dihydroxyphenylglycine or (2S,3S,4S)-α-(carboxycyclopropyl)glycine stimulated [3H]inositolmonophosphate ([3H]InsP) formation in primary cultures of rat hepatocytes. 1S,3R-ACPD-stimulated [3H]InsP formation was inhibited by α-methyl-4-carboxyphenylglycine, indicating that cultured hepatocytes express functional mGlu receptors coupled to polyphosphoinositide hydrolysis. The identity of these receptors is not similar to that of any of the known mGlu receptor subtypes characterized in heterologous expression systems.

KW - Hepatocyte cultured

KW - Metabotropic glutamate receptor

KW - Polyphosphoinositide hydrolysis

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SN - 0014-2999

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JF - European Journal of Pharmacology

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ER -

Metabotropic glutamate receptor agonists stimulate polyphosphoinositide hydrolysis in primary cultures of rat hepatocytes

Abstract

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