TY - JOUR
T1 - Metabonomics study on the effects of the ginsenoside Rg3 in a β-cyclodextrin-based formulation on tumor-bearing rats by a fully automatic hydrophilic interaction/reversed-phase column-switching HPLC-ESI-MS approach
AU - Wang, Yuan
AU - Wang, Jiangshan
AU - Yao, Ming
AU - Zhao, Xinjie
AU - Fritsche, Jens
AU - Schmitt-Kopplin, Philippe
AU - CAI, Zongwei
AU - Wan, Dafang
AU - Lu, Xin
AU - Yang, Shengli
AU - Gu, Jianren
AU - Häring, Hans Ulrich
AU - Schleicher, Erwin D.
AU - Lehmann, Rainer
AU - Xu, Guowang
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/6/15
Y1 - 2008/6/15
N2 - The goal of this study was the application of a novel, fully automatic column-switching approach in a metabonomics study combining the orthogonal selectivities of hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. The temporal, pharmacodynamic effects of the ginsenoside Rg3 on the metabonome in urine of healthy and liver-tumor-bearing rats have been investigated. Within a total analysis time of 52 min we detected 5686 polar, and on the second column an additional 1808 apolar, urinary metabolite ions. The administration of a single, high dose of Rg3 in a β-cyclodextrin-based formulation led to a considerable change of the metabolic pattern in cancer rats during 3 days studied. Seventeen biomarker candidates including three apolar metabolites, which were not retained on the HILIC column, were detected. Overall, the results suggest that the developed liquid chromatography-mass spectrometry strategy is a promising tool in metabonomics studies for global analysis of highly complex biosamples. It may not only increase the number of discovered biomarkers but consequently improve the comprehensive information on metabolic changes in a fully automatic manner.
AB - The goal of this study was the application of a novel, fully automatic column-switching approach in a metabonomics study combining the orthogonal selectivities of hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. The temporal, pharmacodynamic effects of the ginsenoside Rg3 on the metabonome in urine of healthy and liver-tumor-bearing rats have been investigated. Within a total analysis time of 52 min we detected 5686 polar, and on the second column an additional 1808 apolar, urinary metabolite ions. The administration of a single, high dose of Rg3 in a β-cyclodextrin-based formulation led to a considerable change of the metabolic pattern in cancer rats during 3 days studied. Seventeen biomarker candidates including three apolar metabolites, which were not retained on the HILIC column, were detected. Overall, the results suggest that the developed liquid chromatography-mass spectrometry strategy is a promising tool in metabonomics studies for global analysis of highly complex biosamples. It may not only increase the number of discovered biomarkers but consequently improve the comprehensive information on metabolic changes in a fully automatic manner.
UR - http://www.scopus.com/inward/record.url?scp=45249120387&partnerID=8YFLogxK
U2 - 10.1021/ac8002402
DO - 10.1021/ac8002402
M3 - Article
C2 - 18465884
AN - SCOPUS:45249120387
SN - 0003-2700
VL - 80
SP - 4680
EP - 4688
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 12
ER -
