TY - JOUR
T1 - Metabonomic study of the effects of different acupuncture directions on therapeutic efficacy
AU - Ju, Liang
AU - Wen, Yan
AU - Yin, Jia
AU - Deng, Shizhe
AU - ZHENG, Jiangang
AU - Wang, Lei
AU - Deng, Haoyue
AU - Hou, Zhiguo
AU - Zhao, Xiao Feng
AU - He, Si
AU - Huang, Linghui
AU - Zhang, Chao
AU - Tian, Guang
AU - Meng, Zhihong
AU - Li, Yubo
N1 - Funding Information:
This study was supported by the National Basic Research Programs of China (973 Program), No. 2010CB530506 .
PY - 2016/1/15
Y1 - 2016/1/15
N2 - Posterior circulation ischemia (PCI) is a common clinical ischemic cerebrovascular disease that can endanger the lives of patients in severe cases. Our previous research found that needling the Fengchi (GB20) acupoint presents a significant effect on PCI and that different acupuncture directions can exert different effects. To investigate the biological mechanism of acupuncture directions, rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabonomic techniques are used to analyze the metabolic profiles of urine samples. The urine samples were obtained from 30 healthy control subjects, 60 PCI patients before and after treatment of different acupuncture directions. Six metabolites, including LPE (22:6), estrone, uric acid, vanillylmandelic acid, N-acetyl-. l-tyrosine, and 4-hydroxyphenylacetylglutamine were identified as potential biomarkers of acupuncture treatment of PCI. Acupuncture treatment of PCI patients significantly changed the levels of these potential biomarkers. Moreover, different acupuncture directions showed different effects on the contents of these biomarkers. These results strongly support the belief that acupuncture direction performs an important function in acupuncture intervention. The findings provide new insights into the mechanism of acupuncture treatment and reveal that acupuncture manipulation results in various curative.
AB - Posterior circulation ischemia (PCI) is a common clinical ischemic cerebrovascular disease that can endanger the lives of patients in severe cases. Our previous research found that needling the Fengchi (GB20) acupoint presents a significant effect on PCI and that different acupuncture directions can exert different effects. To investigate the biological mechanism of acupuncture directions, rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabonomic techniques are used to analyze the metabolic profiles of urine samples. The urine samples were obtained from 30 healthy control subjects, 60 PCI patients before and after treatment of different acupuncture directions. Six metabolites, including LPE (22:6), estrone, uric acid, vanillylmandelic acid, N-acetyl-. l-tyrosine, and 4-hydroxyphenylacetylglutamine were identified as potential biomarkers of acupuncture treatment of PCI. Acupuncture treatment of PCI patients significantly changed the levels of these potential biomarkers. Moreover, different acupuncture directions showed different effects on the contents of these biomarkers. These results strongly support the belief that acupuncture direction performs an important function in acupuncture intervention. The findings provide new insights into the mechanism of acupuncture treatment and reveal that acupuncture manipulation results in various curative.
KW - Acupuncture
KW - Metabonomics
KW - Posterior circulation ischemia
KW - Rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84949870595&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2015.12.006
DO - 10.1016/j.jchromb.2015.12.006
M3 - Journal article
C2 - 26708629
AN - SCOPUS:84949870595
SN - 1570-0232
VL - 1009-1010
SP - 87
EP - 95
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -
