TY - JOUR
T1 - Metabolomics Deciphered Metabolic Reprogramming Required for Biofilm Formation
AU - Lu, Haitao
AU - Que, Yumei
AU - Wu, Xia
AU - Guan, Tianbing
AU - Guo, Hao
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China Grants (Nos 81274175 and 31670031), National Key R&D Program of China (Nos 2017YFC1308600 and 2017YFC1308605), and the Startup Funding for Specialized Professorship Provided by Shanghai Jiao Tong University (No. WF220441502).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/9
Y1 - 2019/9
N2 - Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UTI89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the dissociation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance.
AB - Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UTI89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the dissociation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance.
UR - http://www.scopus.com/inward/record.url?scp=85072123144&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-49603-1
DO - 10.1038/s41598-019-49603-1
M3 - Journal article
C2 - 31511592
AN - SCOPUS:85072123144
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
M1 - 13160
ER -