Metabolite profiling of tissues of Acorus calamus and Acorus tatarinowii rhizomes by using LMD, UHPLC-QTOF MS, and GC-MS

Yogini Jaiswal, Zhitao LIANG, Alan Ho, Hubiao CHEN, Zhongzhen ZHAO*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

20 Citations (Scopus)

Abstract

Acorus calamus and its related species are of significant importance to the food and fragrance industries due to their varied applications. They are also a cause of critical concern due to their toxic β-asarone content. Several toxicity cases have occurred due to high β-asarone compositions in food products. Hence, limits for their use are strictly regulated by the Food and Drug Administration, the European Union, and legislations of different countries. The identification of species with a lower β-asarone content is of great significance. In this report, the metabolite profiles and essential oil content of A. calamus and Acorus tatarinowii rhizomes were analysed and compared using UHPLC-QTOF-MS and GC-MS techniques. The metabolite profiles were similar; however, β-asarone content was higher in A. calamus rhizomes. The developed methods can be applied for microscopic and macroscopic identification, and quality control of food products containing β-asarone.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalPlanta Medica
Volume81
Issue number4
DOIs
Publication statusPublished - Mar 2015

Scopus Subject Areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

User-Defined Keywords

  • Acoraceae
  • Acorus calamus
  • Acorus tatarinowii
  • GC-MS
  • laser micro-dissection (LMD)
  • metabolite profiling
  • UHPLC-QTOF MS
  • α - and β -asarone

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