TY - JOUR
T1 - Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - A pilot study
AU - Zhang, Xiaojun
AU - Choi, Franky F.K.
AU - Zhou, Yan
AU - LEUNG, Fung Ping
AU - Tan, Shun
AU - LIN, Shuhai
AU - Xu, Hongxi
AU - JIA, Wei
AU - Sung, Joseph J.Y.
AU - CAI, Zongwei
AU - BIAN, Zhaoxiang
N1 - Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - The incidence of inflammatory bowel disease, a relapsing intestinal condition whose precise etiology is still unclear, has continually increased over recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of inflammatory bowel disease. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) was performed to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid. Acquired metabolic profile data were processed by multivariate data analysis for differentiation and screening of potential biomarkers. Five metabolites were identified in urine: two tryptophan metabolites [4-(2-aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12α-hydroxy-3-oxocholadienic acid. Seven metabolites were identified in plasma: three members of the bile acid ? alcohol group (cholic acid, 12a-hydroxy-3-oxocholadienic acid and cholestane- 3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPC(20:4), LysoPC( 16:0), LysoPC(18:1) and LysoPC(18:0)]. These metabolites are associated with damage of the intestinal barrier function, microbiota homeostasis, immune modulation and the inflammatory response, and play important roles in the pathogenesis of inflammatory bowel disease. Our results positively support application of the metabonomic approach in study of the pathophysiological mechanism of inflammatory bowel disease.
AB - The incidence of inflammatory bowel disease, a relapsing intestinal condition whose precise etiology is still unclear, has continually increased over recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of inflammatory bowel disease. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) was performed to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid. Acquired metabolic profile data were processed by multivariate data analysis for differentiation and screening of potential biomarkers. Five metabolites were identified in urine: two tryptophan metabolites [4-(2-aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12α-hydroxy-3-oxocholadienic acid. Seven metabolites were identified in plasma: three members of the bile acid ? alcohol group (cholic acid, 12a-hydroxy-3-oxocholadienic acid and cholestane- 3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPC(20:4), LysoPC( 16:0), LysoPC(18:1) and LysoPC(18:0)]. These metabolites are associated with damage of the intestinal barrier function, microbiota homeostasis, immune modulation and the inflammatory response, and play important roles in the pathogenesis of inflammatory bowel disease. Our results positively support application of the metabonomic approach in study of the pathophysiological mechanism of inflammatory bowel disease.
KW - Experimental inflammatory bowel disease
KW - Metabonomics
KW - Ultra-performance liquid chromatography time-of-flight mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84862580679&partnerID=8YFLogxK
U2 - 10.1111/j.1742-4658.2012.08612.x
DO - 10.1111/j.1742-4658.2012.08612.x
M3 - Journal article
C2 - 22520047
AN - SCOPUS:84862580679
SN - 1742-464X
VL - 279
SP - 2322
EP - 2338
JO - FEBS Journal
JF - FEBS Journal
IS - 13
ER -