TY - JOUR
T1 - Metabolic fate of environmental chemical triclocarban in colon tissues
T2 - roles of gut microbiota involved
AU - Wang, Guangqiang
AU - Zhang, Hongna
AU - Zhang, Jianan
AU - Sanidad, Katherine Z.
AU - Yeliseyev, Vladimir
AU - Parsonnet, Julie
AU - Haggerty, Thomas D.
AU - Yang, Haixia
AU - Ai, Lianzhong
AU - Xie, Minhao
AU - Cai, Zongwei
AU - Zhang, Guodong
N1 - This research is supported by a new faculty start-up from the University of Massachusetts Amherst , USDA NIFA 2019-67017-29248 and 2020-67017-30844 , and USDA/Hatch MAS00556 (to G. Zhang), General Research Fund ( 12303319 ) of Hong Kong Research Grants Council (to Z. Cai), and NIH/NIEHS R21 ES023371 (to J. Parsonnet).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/9/15
Y1 - 2021/9/15
N2 - Metabolic transformations play critical roles in the bioavailability and toxicities of environmental pollutants and toxicants. However, most previous research has focused on the metabolic reactions in host tissues, the gut microbiota-mediated biotransformation of environmental compounds is understudied. Using triclocarban (TCC) as a model environmental compound, here we study the metabolic fate of TCC in gut tissues and determine the roles of gut microbiota involved. We find that compared with other tissues, the colon tissue has a unique metabolic profile of TCC, with high abundance of the parent compound TCC and its free-form metabolites. Using a variety of approaches including antibiotic-mediated suppression of gut bacteria in vivo, germ-free mice, and in vitro culture of fecal bacteria, we found that the unique metabolic profile of TCC in the colon is mediated by the actions of gut microbiota. Overall, our findings support that gut microbiota plays important roles in colonic metabolism of TCC, highlighting the importance to consider the contributions of gut microbiota in toxicology evaluation of environmental compounds.
AB - Metabolic transformations play critical roles in the bioavailability and toxicities of environmental pollutants and toxicants. However, most previous research has focused on the metabolic reactions in host tissues, the gut microbiota-mediated biotransformation of environmental compounds is understudied. Using triclocarban (TCC) as a model environmental compound, here we study the metabolic fate of TCC in gut tissues and determine the roles of gut microbiota involved. We find that compared with other tissues, the colon tissue has a unique metabolic profile of TCC, with high abundance of the parent compound TCC and its free-form metabolites. Using a variety of approaches including antibiotic-mediated suppression of gut bacteria in vivo, germ-free mice, and in vitro culture of fecal bacteria, we found that the unique metabolic profile of TCC in the colon is mediated by the actions of gut microbiota. Overall, our findings support that gut microbiota plays important roles in colonic metabolism of TCC, highlighting the importance to consider the contributions of gut microbiota in toxicology evaluation of environmental compounds.
KW - Biotransformation
KW - Gut microbiota
KW - Metabolism
KW - Triclocarban (TCC)
UR - http://www.scopus.com/inward/record.url?scp=85105795682&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2021.147677
DO - 10.1016/j.scitotenv.2021.147677
M3 - Journal article
C2 - 34004538
AN - SCOPUS:85105795682
SN - 0048-9697
VL - 787
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 147677
ER -